Invitrogen Qubit 2.0 - Guidelines for Using the Qubit 2.0 Fluorometer; Assay Tubes Requirements

13
Guidelines for Using the Qubit
®
2.0 Fluorometer
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To obtain the best results, follow the recommendations below.
For more information, see Critical Qubit
™
Assay Considerations
in the Appendix, pages 33–35.
 Do not operate
the instrument in direct sunlight.
 Wear gloves during sample handling.
 Use the Qubit
®
2.0 Fluorometer at room temperature only
(22–28ºC).
 Store all kit reagents at room temperature and insert all
assay tubes into the Qubit
®
2.0 Fluorometer only for as much
time as it takes for the instrument to measure the
fluorescence.
 Do not hold the assay tubes in your hand before performing
a measurement.
 Make sure that you have calibrated the Qubit
®
2.0
Fluorometer using the appropriate standards.
 Incubate the tubes for the Qubit
™
DNA and RNA assays for
2 minutes after mixing the sample or standard with the
working solution.
 Incubate the tubes for the Qubit
™
protein assays for 15
minutes after mixing the sample or standard with the
working solution.
 If you are performing multiple readings of a single tube,
remove the tube from the instrument and let it equilibrate to
room temperature for 30 seconds before taking another
reading.
 Visit www.invitrogen.com/qubit for additional application
notes, technical notes, citations, firmware updates, and a list
of validated Qubit
™
assays that have been tested using the
Qubit
®
2.0 Fluorometer.
Assay Tubes
for the
Qubit
®
2.0
Fluorometer
Only thin-wall, clear 0.5 mL PCR tubes are appropriate for use in
the Qubit
®
2.0 Fluorometer. Acceptable tubes include Qubit
®
assay
tubes (Cat. no. Q32856, 500 tubes) or Axygen PCR-05-C tubes
(VWR, part number 10011-830). The minimum assay volume must
be 200 L for an accurate read.