33
Appendix
Critical Qubit
™
Assay Considerations
How the
Qubit
®
2.0
Fluorometer
Calculates
Concentration
The Qubit
®
2.0 Fluorometer generates concentration data based on
the relationship between the two Standards used in calibration
(three for the Qubit
™
protein assay). The plot below shows the line
corresponding to the curve-fitting algorithm (a modified Hill plot)
used in the calculation of concentration data for the Qubit
™
RNA
assay. For reference, the positions of the Standards and a set of data
points from an actual experiment are shown superimposed onto the
line. This plot demonstrates that the curve-fitting algorithm gives
accurate values for quantitation.
Figure 1. The curve-fitting algorithm used to determine concentration in the
Qubit
™
RNA assay. Data for the Qubit
™
dsDNA BR, Qubit
™
dsDNA HS,
Qubit
™
ssDNA, Qubit
™
RNA BR, and Qubit
™
protein assays are generated
by similar algorithms.
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