Invitrogen Qubit 2.0 - Reading Samples; Performing Sample Readings

19
Reading Samples
Introduction
After you have completed the calibration of the Qubit
®
2.0
Fluorometer by running the appropriate standards for the assay
you want to use or have accepted the values from the previous
calibration, you are ready to read your samples. This section
provides instructions for using the Qubit
®
2.0 Fluorometer to
quantitate your DNA, RNA, or protein samples with the
appropriate Qubit
™
assay.
It is important to prepare the sample correctly before a reading is
taken. For instructions on the preparation of your samples, see the
instructions that accompany the assay you are using.
Materials
Needed
 Qubit
™
assay kit you want to use to quantitate your samples
(see page 33 for available Qubit
™
assay kits and ordering
information)
 Your DNA, RNA, or protein samples
Note: Incubate the samples for the appropriate amount of time after
mixing them with the working solution (2 minutes for the Qubit
™
DNA and RNA assays, 15 minutes for the Qubit
™
protein assay).
 Qubit
®
assay tubes or other appropriate 0.5 mL assay tubes
 Optional: Qubit
®
2.0 USB drive for data transfer, supplied with
the instrument or available separately
Reading
Samples
1. Choose Sample to go to the Sample Screen.
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