Invitrogen Qubit 2.0 - Running New Calibration Standards (Continued)

16
Calibrating the Qubit
®
2.0 Fluorometer, Continued
Running New
Standards for
Calibration,
continued
4. Insert Standard #1 into the Sample Chamber and press Read.
Ensure that you are using the Standard #1 appropriate for the
assay you are performing. The reading takes approximately
3 seconds.
5. Insert Standard #2, and press Read. Ensure that you are using
the Standard #2 appropriate for the assay you are performing.
6. If you are performing the Qubit
™
protein assay, which
requires a three-point calibration, insert Standard #3 when
prompted, and then press Read.
The calibration is complete after Standard #2 is read (or after
Standard #3 is read for the Qubit
™
protein assay). The new
standards graph with data points for standards connected by
a line appears on the screen.
Continued on next page