NITROGEN, TOTAL
CHROMOTROPIC ACID WITH PERSULFATE
DIGESTION METHOD • CODE 4026-01
QUANTITY CONTENTS CODE
25 Total Nitrogen Hydroxide Reagent Tubes 4040-G
5 g *Digestion Reagent Powder *4036-C
60 mL Deionized Water *5115PS-H
5 g *Total Nitrogen Reagent A Powder *4041-C
30 *Total Nitrogen Reagent B Tablets *4042A-G
25 *Total Nitrogen Acid Reagent Tubes *4043-G
2 Spoon, 0.15 g, plastic 0727
4 Pipets, 1.0 mL, plastic 0354
2 Funnels, plastic 0459
Note: for greater accuracy, use laboratory grade pipets.
Equipment needed but not supplied:
1 COD Adapter 5-0087
1 COD Reactor, 12 tube, 110V 5-0102
or 1 COD Reactor, 12 tube, 230V 5-0102-EX2
1 Measuring Pipet, 1.0 mL 2-2110
1 Pipet Bulb 2-2164
1 Wipes 2-2069
1 Test Tube Holder 2-2190
Nitrogen is essential for plant growth, but the presence of excessive amounts in
water supplies presents a major pollution problem. Nitrogen compounds may enter
water as nitrates or be converted to nitrates from agricultural fertilizers, sewage,
industrial and packing house wastes, drainage from livestock feeding areas,
farm manures and legumes. Nitrates in large amounts can cause “blue babies”
(methemoglobinemia) in infants less than six months of age. Nitrate concentration
is an important factor to be considered in livestock products, where, in addition to
causing methemoglobinemia, it is responsible for many other problems. Nitrates
SMART3 Test Procedures 2.11 NITROGEN, Total
Test Procedures
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