• Save your new settings under a different name and load them for imaging
• Set up your experiment, press start
• The deconvolution will start immediately online, when you have started your
experiment
• Raw data and deconvolved image will be saved as separate files in Open
project
Additional info@:
file:///C:/Program%20Files/Leica%20Microsystems%20CMS%20GmbH/LAS%20X/BIN/UserH
elp/UserHelpEnglish/Default.htm#DLG/Wizards/DLG_lightning_lightning_grade.htm
• Deconvolution processing after imaging in standard mode
• Images taken in the regular confocal environment may be deconvolved
afterwards
• select the image in Open project, then go to the Process menu→ Lightning
• put in your parameters and press “Apply”
• Switching off the system:
• Switch off lasers in the LASX software ‘Configuration’-‘lasers’ (The Laser is not off
if you just close the software!!)
• Remove your files from the project list (the next user might write data in your folder
or delete your data accidentally)
• Data upload is triggered by Log off/Log on, data will be uploaded to the following
folder
\\scratch3.ist.local\scratch-bioimaging\{Your group Name}\_ImageDrop\
For manual upload, map the above path. login your usermane should be inserted as :
IST\username
• Shut down LAS AF, shut down the computer.
• Turn the ‘Laser emission’ key (4) to the ‘off’ position
• Then switch off ‘Laser Power’ (3), ‘Scanner Power’ (2) and ‘PC/Microscope’ (1).
• Switch off the fluorescent lamp (5)
To Next Page
