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MRC INE-PHS-3E - Buffer Solution Preparation

MRC INE-PHS-3E
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INE-PHS-3E 2016
14
Appendix 4: Way to make buffer solution
1. pH 4.00 solution: Dissolve 10.12g GR potassium acid phthalate dissolved in 1000ml
high-purity deionized water.
2. pH 6.86 solution: Dissolve 3.387g GR monopotassium phosphate and 3.533g GR
disodium hydrogen phosphate in 1000ml high-purity deionized water.
3. pH 9.18 solution: Dissolve 3.80g GR sodium tetraborate into 1000mL high-purity
deionized water.
NOTE˖The water used for configuring solution said above should be boiled for 15~3
0 min to get rid of the dissolved CO2. It also should be kept from air to avoid pollut
ion.

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