Chapter 1 Microscopy Procedures
6
Chapter 1-1
Microscopy Procedures
Bright-field Microscopy
9
Focus and center the condenser. (See Chapter 2, “5 Focusing and Centering the Condenser” for details)
(1) Look into the eyepiece with the field diaphragm
stopped down to the minimum. Focus on the field
diaphragm image using the condenser focus knob,
then adjust the condenser centering screws to
center the diaphragm image within the field of view.
(2) Bring the 40x objective into the optical path to check
the focus and centering of the field diaphragm
image. Make adjustments in the same way as step
(1) as necessary.
0.8
0.6
0.4
0.2
(3) Turn the field diaphragm dial and adjust the field
diaphragm image so that its size is almost the same
as the field of view.
Adjusting aperture diaphragm
Field diaphragm
image
Eyepiece field
of view
Field diaphragm
image
Eyepiece field
of view
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