Nikon eclipse Ci-L User Manual

Chapter 1 Microscopy Procedures
28
Chapter 1-5
Microscopy Procedures
Epi-fluorescence Microscopy
5.2
Epi-fluorescence Microscopy Procedure
WARNING
The light source used with the epi-fluorescence attachment (mercury lamp) requires special care during handling
because of its characteristics. Make sure you are familiar with and adhere to all warnings and cautions described at
the beginning of this instruction manual.
Locate the observation target on the specimen under bright-field microscopy, then proceed to epi-fluorescence
microscopy.
(See Chapter 2, “15 Tips for Epi-fluorescence Microscopy” for the tips to locate the observation target on the specimen.)
1. Turn off the dia-illumination power.
2. Close the shutter.
3. Bring the filter cube into the optical
path.
4. Fully open the field diaphragm.
5. Turn on the mercury lamp.
6. Open the shutter.
7. Bring the desired objective into the
optical path.
8. Focus on specimen.
9. Circumscribe field diaphragm to
field of view.
10. View specimen.
11. Turn off the mercury lamp.
CLAMP
TORQUE
ND4
ND8
OUT
IN
INTENSILIGHT
C
-
HGFI
LAMP
ND
1
2
4
32
16
8
SHUTTER
POWER
RUN TIME hrs.
A
1
-
2
-
3
-
4
B1
-
2 / 3
-
4
C1
-
2
-
3
-
4
1
1
4
2
3
4
CUBE
Microscopy operation (→See also: Chapter 2, “15 Tips for Epi-fluorescence Microscopy”)
1
Turn off the microscope power switch (turn off the dia-illumination).
Turn off the power switch (press to the “O” position) for the
microscope. (The power LED on the front of the main
body will turn off.)
ND4
ND8
O
UT
IN
Turning off the microscope
8
10
3
2 6
1
7
5
11
9
4

Nikon eclipse Ci-L Specifications

General

Brand	Nikon
Model	eclipse Ci-L
Category	Microscope
Language	English