Nikon eclipse Ci-L - Table of Contents

Table of Contents
iv
Table of Contents
Introduction .........................................................................................................................i
Contents of the Manual ....................................................................................................ii
Symbols Used in This Manual..........................................................................................ii
Table of Chapters.............................................................................................................. iii
Safety Precautions............................................................................................................ vi
WARNING and CAUTION Symbols used in This Manual...............................................vi
Meaning of Symbols Used on the Product......................................................................vi
WARNING ................................................................................................................vii
CAUTION ..................................................................................................................ix
Notes on Handling the Product.......................................................................................... x
Microscopy Procedures.......................................................................... 1
1 Bright-field Microscopy..............................................................................................1
1.1 System Configuration and Controls ................................................................... 1
1.2 Bright-Field Microscopy Procedure.................................................................... 2
2 Phase Contrast Microscopy ......................................................................................9
2.1 System Components and Controls .................................................................... 9
2.2 Phase Contrast Microscopy Procedure ........................................................... 10
3 Simple Polarizing Microscopy .................................................................................15
3.1 System Configuration and Controls ................................................................. 15
3.2 Simple Polarizing Microscopy Procedure ........................................................ 16
4 Sensitive Tint Plate Microscopy ..............................................................................21
4.1 System Configuration and Controls ................................................................. 21
4.2 Sensitive Tint Plate Microscopy Procedure...................................................... 22
5 Epi-fluorescence Microscopy ..................................................................................27
5.1 System Configuration and Controls ................................................................. 27
5.2 Epi-fluorescence Microscopy Procedure ......................................................... 28
Individual Operations ............................................................................ 33
1 Adjusting the Brightness of a Diascopic Image.......................................................33
1.1 Adjustment with the Dia-illumination Brightness Control Knob........................ 33
1.2 Adjustment with the ND Filter (for Ci-S)........................................................... 33
1.3 Removing an NCB filter for a brighter image (Ci-S)......................................... 34
2 Focusing on the Specimen (Vertical Stage Movement)...........................................35
2.1 Focus Knob Rotation and Stage Movement .................................................... 36
2.2 Number of Focus Knob Turns and Distance of Stage Travel........................... 36
2.3 Adjusting the Rotating Torque of the Coarse Focus Knob............................... 37
2.4 Refocusing ....................................................................................................... 37
2.5 Position Exchange of the Fine Focus Knob ..................................................... 38
3 Bringing the specimen into the optical path (Horizontal Stage Movement).............39
3.1 Knob Rotation and Stage Movement ............................................................... 39
3.2 Adjusting the Knob Heights.............................................................................. 39
3.3 Adjusting the Knob Rotation Torque................................................................. 39
4 Adjusting the Diopter...............................................................................................40
5 Adjusting aperture diaphragm .................................................................................41
6 Adjusting the Aperture Diaphragm ..........................................................................42
6.1 Adjusting the Aperture Diaphragm Using the Condenser Scale...................... 42
6.2 Adjusting the Aperture Diaphragm Using the Centering Telescope................. 42
7 Selecting a Condenser............................................................................................43
8 Adjusting the field diaphragm..................................................................................43