Chapter 1 Microscopy Procedures
8
Chapter 1-1
Microscopy Procedures
Bright-field Microscopy
13
Adjust the field diaphragm
Turn the field diaphragm dial to adjust the field diaphragm
so that it almost circumscribes the field of view.
Size of the field diaphragm
Normally, adjust the field diaphragm so that it
almost circumscribes the field of view. Opening
the field diaphragm too much results in stray light
entering the field of view, generating flare and
reducing the image contrast. In addition, the
specimen will become decolorized over a wide
area.
Field diaphragm's adjustment timing
Be sure to adjust the field diaphragm each time
you change the objective.
Circumscribe around the field of view
Adjusting the field diaphragm
14
View the specimen.
Rotate the stage knob to move the target. If the target is
not in focus, use the focus knob to adjust the focus.
15
Turn off the power.
Turn off the power switch (press to the “O” position) for the
microscope. (The power LED on the front of the main
body will turn off.)
ND4
ND8
UT
IN
Power off
POWER
Power LED off
OFF
Field of view
Field Diaphragm
Field diaphragm
dial
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