Chapter 1 Microscopy Procedures
13
Chapter 1-2
Microscopy Procedures
Phase Contrast Microscopy
13
Bring an arbitrary Ph objective into the optical
path.
Turn the nosepiece to bring the desired Ph objective into the optical path.
When using an oil immersion type objective, apply immersion oil between the specimen and the objective.
(→Chapter 2 “12 Oil Immersion” for details)
Phase turret condenser
The phase turret condenser is intended to be used dry. DO NOT apply immersion oil between the condenser
tip's ball and the specimen.
14
Adjust the Ph annular diaphragm in the condenser with the Ph objective to be used.
Turn the condenser turret to bring an annular diaphragm
with the same Ph code as the objective into the optical
path.
CLAMP
TORQUE
PHASE CONTRAST
0.90 DRY
JAPAN
A
Matching the Ph codes of Ph annular
diaphragm and the Ph objective
Ph code
One of the Ph codes, [Ph1], [Ph2], or [Ph3] is indicated on the Ph objective depending on the size of the phase
plate. (Ph codes have nothing to do with the magnification of the objective.) Always use a Ph objective and Ph
annular diaphragm with the same Ph code. You cannot experience the phase effect if a different combination
of the codes might be used.
Centering of the annular diaphragm and the phase plate
The position of each annular diaphragm in the condenser turret has already been adjusted based on the Ph1
annular diaphragm, but the phase image will differ slightly depending on how the annular diaphragm overlaps
the phase plate. For a stricter observation or capturing of still images, check whether the annular diaphragm
and the phase plate are concentric at each magnification.
15
Focus on the specimen.
(1) Look into the eyepiece, and adjust the brightness of the field of view by turning the dia-illumination brightness
control knob. You can also adjust the brightness with an ND filter for Ci-S.
(2) Rotate the stage knob to move the stage and bring the target into the optical path.
(3) If the specimen is not in focus, turn the focus knob to focus on it.
Same Ph code
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