Chapter 4 Troubleshooting
70
Chapter 4
Troubleshooting
Problem Cause Measure
The nosepiece is not attached correctly, or
has not been fully rotated to the click stop
position.
Attach it correctly and rotate to the click stop
position.
(→Chapter 3, “2 Assembly for Bright-field
Microscopy ― 6 Attaching the nosepiece”)
The specimen is tilted relative to the stage
surface.
Position the specimen in place on the stage.
(→Chapter 2 “2.1 Bright-field Microscopy Procedure
- 5 Place a specimen on the stage, and move the
stage to bring the target into view”)
The stage is tilted.
Attach the stage correctly.
(→Chapter 3, “2 Assembly for Bright-field
Microscopy ― 2 Attaching the stage”)
One side of the field of
view (up, down, right, or
left) is not focused.
The image flows (i.e.
becomes asymmetrically
defocused when moving
the focal point)
The condenser is tilted.
Attach the condenser correctly.
(→Chapter 3, “2 Assembly for Bright-field
Microscopy ― 3 Attaching the condenser”)
Interpupillary adjustment has not been
performed.
Perform interpupillary adjustment.
(→Chapter 2 “2.1 Bright-Field Microscopy
Procedure ― 8 Adjust the interpupillary distance”)
Images in left and right
eyepieces are not
coincident.
Diopter adjustment has not been
performed.
Perform diopter adjustment.
(→See Chapter 2 “4 Adjusting the Diopter” in the
“Operation”.)
Diopter adjustment has not been
performed.
Perform diopter adjustment.
(→See Chapter 2 “4 Adjusting the Diopter” in the
“Operation”.)
Eyes become fatigued.
Brightness is inadequate.
Adjust the brightness using the brightness
control knob or ND filters to attain a suitable
brightness.
(→Chapter 2, “1 Adjusting the Brightness of a
Diascopic Image”)
The specimen holder is not securely-fixed
on the stage.
Fix the holder securely.
(→Chapter 2 “2.1 Bright-field Microscopy Procedure
- 5 Place a specimen on the stage, and move the
stage to bring the target into view”)
The specimen does not
move smoothly.
Rotating torque of the stage knob is set too
heavy.
Adjust to appropriate torque weight.
(→Chapter 3 “3.3 Adjusting the Knob Rotation
Torque”)
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