Nikon Eclipse E400 POL User Manual

Switch to any desired objective 3
and view the specimen. / B Switch the objective.
Rotate the revolving nosepiece to
its click-stop position.
In the case of using an oil-
immersion objective, apply oil Ё
while being careful that air bubbles
do not form between the end of the dd
objective and the specimen.
Manipulate the coarse focus knob
or fine focus knob to re-focus on
the specimen.
E Readjust the focus.
~
Close the field diaphragm so that it
is
just
outside
the
field
of
view.
Ell
Adjust
the
condenser
(p.20) aperture diaphragm.
| Close the condenser aperture
diaphragm to about 70 — 80% of
| the numerical aperture of the
* objective. (p.19)
EJ Adjust the field diaphragm.
This completes the procedure for microscopy for normal observation. Refer to the following for
details regarding the microscopy procedures for "Orthoscopic Observation" and "Conoscopic
Observation".
12

Nikon Eclipse E400 POL Specifications

General

Brand	Nikon
Model	Eclipse E400 POL
Category	Microscope
Language	English