Nikon Optiphot - Care for Starting Repair

Digital Microsopy Center, University of Washington
July 29, 2016 4
4. Select your objective lens
4.1. Choose the objective lens compatible to your specimen and needs
4.1.1. low NA will provide low resolution and weak fluorescence, but large working
distance
4.1.2. low magnification providse large field of view but may not magnify sufficiently
for full resolution at the camera
Locking screw for stage rotation.
Condenser diaphragm (NA adjustment)
Centering screws for condenser alignment.
Lever to flip condenser top lens in or out of optical path.
Stage Y-axis motion
Stage X-axis motion
Figure 2. Sub-stage Condenser