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Nikon Optiphot - Page 7

Nikon Optiphot
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Digital Microscopy Center, University of Washington
July 29, 2016 7
Table 3. Epi-fluorescence filter choices
*UV capable, coverslip correction collar
**Variable NA 0.8-1.3
Table 4. Spatial calibration for each objective lens, with the Spot IIe camera.
Objective lens notes.
Other objective lenses are available upon request, such as 60/1.4 oil, 100/1.25 oil.
Lenses designated as “160/-” do not require a coverslip.
Lenses designated as “160/.17” require a coverslip of 170 µm thickness.
Filter Label
Cube Label
“Color”
Part
Nikon
FITC#
HQ:FITC#
green#
96170#
C01174#
TxR#
HQ:TXRD#
red#
96172#
C00178#
UV#
UV-2E/C#
blue#
blue#
#
Cy5#
Cy5#
far#red#
31023#
C81736##
Lens
µm/pixel
pixel/µ
m
1 mm reticle
field
1/.04 Plan 160/-
8.40
0.12
2/.05 Plan 160/-
4.24
0.24
4/.1 EPlan 160/-
2.12
0.47
10/.40 EPlan 160/-
0.87
1.15
820 µm
2
20/.40 EPlan 160/-
0.43
2.31
412 µm
2
40/.65 EPlan 160/.17
0.21
4.78
200 µm
2
40/.85 Fluor 160/.17*
0.21
4.78
200 µm
2
100/1.30 Oil Fluor 160/.17**
0.09
11.55

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