Nikon TE2000 Instructions

2
- The fluorescence filter wheel located underneath the objective wheel should be on
the empty position indicated by the double arrow symbol (↔).
- Place the culture vessel or glass slide on the stage.
- Move a low power objective (e.g. 4x or 10x) into the light path.
- Focus on the specimen. Adjust the condenser to the correct height for Koehler
illumination
1
. Ask core staff for help with this if necessary.
- Have the phase ring set to A for stained cells/tissue, or to Ph1 for phase contrast
when using the 4x, 10x and 20x objectives or to Ph2 for phase when using the 40x
and 60x objectives.
- If microscope slides are to be viewed, they can be placed with the cover-slip side
either up or down [with the 60x objectives place slides cover-slip down].
Using the 20x, 40x and 60x objectives
- Focus using a low power 4x or 10x objective and the coarse focusing wheel. Then
revolve to the 20x, 40x or 60x objective as required.
- Please take extra care to avoid sharp impacts when switching
between objectives.
- Adjust the 20x, 40x or 60x objective correction collar for the optimal resolution
2
.
- A 60x oil objective and a 1x air objective are also available if required. These
must be fitted by the Core staff. If you need to use either of these objectives,
please ask a member of core staff to set up the microscope correctly for you
1
With multi-well plates Koehler illumination may not be possible as the condenser might have to
be moved higher to prevent it hitting the top of the plate. There are twin white markers on the
condenser to show the approximate position for Koehler illumination.
2
With the 20x, 40x and 60x LD air objectives the correction collar should be set to the thickness
of the support material: e.g. approx. 2mm for slides (cover-slip up) or 0.17mm for the thickness
of a 0.17mm cover-slip (cover-slip facing down). The correction collars compensate for
differences in vessel base or slide/cover-slip thickness. The objective collars can be adjusted by
rotating them, and used in conjunction with the normal focus, an optimal image can be
obtained. Please ask Core staff for help with adjusting correction collars. Details of the
correction collars are given on the nearby shelf and on-line.

Nikon TE2000 Specifications

General

Type	Inverted Microscope
Optical System	CFI60 infinity optical system
Focus	Coarse and fine focusing
Nosepiece	6-position nosepiece
Eyepiece Tube	Binocular or trinocular eyepiece tubes available.
Compatibility	Compatible with various accessories, including cameras
Stage	Mechanical stage