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Nikon ECLIPSE Ti2-U User Manual

Nikon ECLIPSE Ti2-U
203 pages
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M702
E 21.2.NF.8 (1/2)
*M702EN08*
Inverted Research Microscope
Instruction Manual

Table of Contents

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Nikon ECLIPSE Ti2-U Specifications

General IconGeneral
BrandNikon
ModelECLIPSE Ti2-U
CategoryMicroscope
LanguageEnglish

Summary

Safety Precautions

Meaning of Symbols Used on the Product

Explains symbols found on the product to indicate hazards and precautions.

WARNING and CAUTION Symbols Used in This Manual

Explains the meaning of WARNING and CAUTION symbols used throughout the manual.

Handling of This Product

Handle with care

Emphasizes careful handling to avoid damage to precision optical components.

Installation location and storage location

Specifies environmental and location requirements for installation and storage.

Nosepiece protection

Instructions for protecting the nosepiece from dust and light.

Oil-immersion microscopy

Guidance on using immersion oil correctly to avoid issues during microscopy.

Chapter 1 Names of Parts and Their Functions

1.1 System Configuration and Names of Components

Details the basic configuration of the microscope and names its components.

1.2 Nomenclature and Functions

Explains the names and functions of various microscope parts and controls.

1.3 Operation Sections of the Microscope Main Body

Covers the main operational controls and sections of the microscope.

Chapter 2 Microscopy

2.1 Diascopic Bright-field (BF) Microscopy

Step-by-step guide for configuring the microscope and performing bright-field microscopy.

2.2 Diascopic Phase Contrast (Ph) Microscopy

Step-by-step guide for setting up and performing phase contrast microscopy.

2.3 Diascopic DIC / IMSI Microscopy

Provides instructions for setting up and performing DIC/IMSI microscopy.

2.4 Episcopic Fluorescence (Epi-FL) Microscopy

Step-by-step guide for setting up and performing epi-fluorescence microscopy.

2.5 Diascopic Dark-field (DF) Microscopy

Step-by-step guide for setting up and performing dark-field microscopy.

2.6 NAMC Microscopy

Step-by-step guide for setting up and performing Nikon Advanced Modulation Contrast microscopy.

Chapter 3 Usage of Components

3.1 Power-related Operation

Explains the procedure for turning the microscope and its peripheral devices on and off.

3.2 Optical Parts in the Microscope Main Body

Details the optical parts of the main microscope body, including output ports.

3.3 Using the Dia-illumination Section

Explains the operation of the dia-illumination section, including shutter and brightness controls.

3.4 Using the Condenser Section

Covers the selection and use of different condenser types and their modules.

3.5 Using the Polarizer and Analyzer

Explains how to attach and use polarizers and analyzers for DIC, NAMC, and IMSI microscopy.

3.6 Using the Tube and the Objective

Covers adjustments related to the microscope tube and objectives for optimal viewing.

3.8 Using the Stage

Covers the procedures for using and setting up the microscope stage.

3.9 Using the Nosepiece

Explains how to switch objectives using the nosepiece and place DIC prisms.

3.10 Using the Objective

Covers using objectives, including adjusting for cover glass thickness and oil immersion.

3.11 Using the Focusing Device

Covers the operation of the microscope's focusing knobs for precise image adjustment.

3.12 Using the FL turret

Covers the operation of the FL turret for switching filter cubes and using its shutter.

3.13 Using the Epi-fluorescence Attachment

Explains how to use the epi-fluorescence attachment, including diaphragm adjustments.

3.14 Using a Back Port Unit (Optional)

Explains how to attach a TI-BPU back port unit for camera devices and its focusing/centering.

3.15 Using a Centering C-mount Adapter

Describes how to use a centering C-mount adapter for focusing and centering camera devices.

Chapter 4 Microscopy Techniques

4.1 Details of Diascopic Bright-field (BF) Microscopy

Explains the principles of bright-field microscopy and its optical system.

4.2 Details of Phase Contrast (Ph) Microscopy

Explains the principles of phase contrast microscopy and how it visualizes phase objects.

4.3 Details of Diascopic DIC and IMSI Microscopies

Explains the principles of DIC and IMSI microscopy, and their optical systems.

4.4 Details of Episopic Fluorescence (Epi-FL) Microscopy

Explains the principles of epi-fluorescence microscopy and its optical system.

4.5 Details of Diascopic Dark-field (DF) Microscopy

Explains the principles of dark-field microscopy and its optical system.

4.6 Details of NAMC Microscopy

Explains the principles of NAMC microscopy and its optical system.

Chapter 5 Troubleshooting

5.1 Troubleshooting for Microscopy Techniques

Lists common troubleshooting steps for microscopy techniques where the image is invisible or limited.

5.2 Troubleshooting for the Units

Provides troubleshooting steps for the microscope main body and dia-illumination section.

Chapter 6 Maintenance and Storage of the Device

6.1 Cleaning the Device

Details the cleaning tools and precautions for cleaning the microscope's lenses and components.

6.2 Storing the Product

Provides precautions for storing the product to prevent mold, condensation, and dust.

Chapter 7 Assembly of the Devices

7.1 Ti2-U System Configuration

Shows a system configuration diagram of the Ti2-U microscope and its various components.

7.2 List of Components

Provides a comprehensive list of all components for the ECLIPSE Ti2-U microscope, including models and remarks.

7.3 Installing the Microscope Main Body (Base)

Provides instructions for installing the microscope main body, including removal and placement.

7.4 Mounting the Tube Base Unit

Explains how to mount the eyepiece tube base unit onto the microscope main body.

7.5 FL Turret (Required for Epi-FL Microscopy)

Describes how to attach the FL turret, which is necessary for epi-fluorescence microscopy.

7.6 Attaching a Nosepiece

Covers attaching objectives to the nosepiece, including notes on handling and protection.

7.7 Mounting a Stage

Covers the procedures for using and setting up the microscope stage.

7.8 Mounting a Diascopic Illumination Pillar and a Lamphouse

Details mounting the diasocpic illumination pillar and lamphouse components.

7.10 Attaching an Analyzer and a Polarizer (Required for DIC Microscopy)

Explains how to attach analyzers and polarizers to the microscope main body for DIC microscopy.

7.11 Attaching a Condenser

Describes procedures for mounting condenser modules, condensers, and condenser lenses.

7.12 Attaching the Tube and Eyepieces

Explains how to attach the eyepiece tube and eyepieces to the binocular part.

7.13 Attaching an Epi-fluorescence Attachment

Covers attaching the FL turret, epi-fluorescence attachment, and light source to the microscope.

7.14 Attaching a Camera Device

Covers attaching camera devices to side ports or back ports of the microscope.

7.15 Connection of Each Cable

Explains how to connect cables for each device, including cautions and connector positions.

7.16 Attaching Objectives

Covers attaching objectives to the nosepiece, including notes on handling and protection.

7.17 Attaching a Specimen Holder

Explains how to attach specimen holders and stage rings to the stage.

7.18 Moving the Microscope

Provides instructions on moving the microscope using TI-BCH carrying handles, including precautions.

Chapter 8 Specifications

8.1 Principles of Operation

Explains the basic principles of microscope operation and its intended purpose and users.

8.2 Performance Properties

Details the performance properties of the Ti2-U microscope main body and AC adapter.

Diascopic illuminator

Lists specifications for the diasocpic illuminator components like pillars, lamphouses, and filter sliders.

Tube base unit

Lists specifications for the Ti2-U tube base units.

Eyepiece tube

Lists specifications for S tube and ER tube eyepiece configurations.

Stage

Lists specifications for different types of stages, including stroke and dimensions.

Nosepiece

Lists specifications for DIC and Sextuple nosepieces.

FL turret

Details specifications for the Intelligent Epi Filter Turret.

Epi-fluorescence attachment

Lists specifications for the fixed main branch, sub branch, and EPI-FL module.

Model

Specifies the model of the microscope as ECLIPSE Ti2-U.

Electrical shock protection class

Indicates the electrical shock protection class of the microscope and AC adapter.

Environmental conditions

Lists operational and storage environmental conditions, including temperature, humidity, and altitude.

External dimensions and mass

Provides external dimensions and mass for the microscope system and main body.

Safety standards

Lists safety standards and compliance information, including CE, EMC, and FCC regulations.

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