Chapter 2 Microscopy
15
Chapter 2 Microscopy
Chapter
2
Microscopy
This chapter explains the procedures for performing the following microscopy techniques in a standard
system:
• Diascopic bright-field (BF) microscopy (☞ 2.1)
• Diascopic phase contrast (Ph) microscopy (☞ 2.2)
• Diascopic differential interference contrast (DIC) / IMSI microscopy (☞ 2.3)
• Episcopic fluorescence (Epi-FL) microscopy (☞ 2.4)
• Diascopic dark-field (DF) microscopy (☞ 2.5)
• Nikon Advanced Modulation Contrast (NAMC) microscopy (☞ 2.6)
System configuration
The microscopy procedure is described based on a microscopy system in the basic configuration described
in “Chapter 1 Names of Parts and Their Functions.”
If any of these microscopy techniques require additional parts or components, they are described on the
pages corresponding to such techniques.
Setup of the system
The following procedures assume that all the necessary devices are mounted, the cable connections have
been completed, and the necessary data for the mounted components is already registered.
If the system has not been assembled yet, assemble the microscope according to the procedure described
in “Chapter 7 Assembly of the Devices.”
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