Nikon ECLIPSE Ti2-U - Troubles in DIC and IMSI Microscopies

Chapter 5 Troubleshooting
110
5.1.4 Troubles in DIC and IMSI Microscopies
Problem Check item
A portion of the field of view is missing.
Condenser
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Turn the condenser turret to the regular position. ( ☞ 3.4.6)
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For an HNA condenser slider, move the slider to the limit position. ( ☞ 3.4.6)
Nosepiece / objective
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Push in the DIC slider until it stops. ( ☞ 3.9.2)
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Correctly attach the nosepiece. ( ☞ 7.6)
There is no contrast in
the DIC or IMSI image.
Polarizer / Analyzer
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Place the polarizer and the analyzer into the optical path. ( ☞ 3.5.1, 3.5.2, 4.3.2)
Nosepiece / objective
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Place the DIC/IMSI objective into the optical path. ( ☞ 3.9.1, 4.3.2)
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Place the DIC slider into the optical path. ( ☞ 3.9.2, 4.3.2)
Condenser module
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Place the DIC/IMSI module into the optical path ( ☞ 3.4.6, 4.3.2)
Combination of optical elements
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Confirm that the combination of the objective, the DIC slider and the condenser
module is correct. (
☞ 4.3.3)
The contrast in DIC or IMSI image is
poor.
Polarizer / Analyzer
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Turn the polarizer rotation lever until a dark cross appears. ( ☞ 3.5.1)
Combination of optical elements
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Select the combination of the optical elements suitable for the specimen. ( ☞ 4.3.3)
Specimen / stage
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Check whether plastic vessels or lids are used. ( ☞ 4.3.1)
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Use a glass slide and a cover glass free of distortion, dust, or dirt. ( ☞ 4.3.1)
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When a combination of an oil-immersing objective and a heat plate are used for
IMSI microscopy, a heat plate with a hole must be used.
The total thickness of the heat plate and the cover glass must be within the
supporting range (0.6 to 1.3 mm) of the correction collar.