Nikon ECLIPSE Ti2-U User Manual

Chapter 4 Microscopy Techniques
81
Advantages of BF Microscopy
• Because specimens can be illuminated brightly, this microscopy is suitable for stained specimens. This
microscopy is also appropriate for focusing or searching for observation objects as a preparatory step for
other microscopies.
• The contrast of the specimen can be adjusted by changing the diameter of the aperture diaphragm.
Disadvantages of BF Microscopy
• BF microscopy is not suitable for observing colorless, transparent specimens that do not absorb light.
4.1.2 Optical Elements Required for BF Microscopy
Dia-illumination unit
TI2-D-PD dia-illumination pillar is used.
S.
F.
C
O
TI2-D-PD Pillar for Dia Illumination
Light source
The following two types are usable as a light source for dia illumination.
• TI2-D-LHLED LED Lamp House for Dia Illumination
• D-LH/LC Precentered Lamphouse
TI2-D-LHLED LED Lamp House for Dia
Illumination
D-LH/LC Precentered Lamphouse
Light source for dia-illumination
Objective
All CFI-60 objectives for inverted microscope allow BF microscopy.

Nikon ECLIPSE Ti2-U Specifications

General

Brand	Nikon
Model	ECLIPSE Ti2-U
Category	Microscope
Language	English