Chapter 4 Microscopy Techniques
82
Condenser lens
Condenser lenses that allow BF microscopy are as follows:
LWD condenser lens ELWD condenser lens CLWD condenser lens HNA condenser lens (dry) HNA condenser lens (oil)
Usable condenser lens
LWD condenser
lens
ELWD condenser
lens
CLWD condenser
lens
HNA condenser
lens (dry)
HNA condenser
lens (oil)
NA 0.52 0.3 0.72 0.85 1.35
Working
distance
30 mm 75 mm 13 mm 5 mm
1.9 mm
(including the glass
slide thickness of
1.2 mm)
Condenser
module
OPEN
(without module)
OPEN
(without module)
OPEN
(without module)
OPEN
(without module)
OPEN
(without module)
Other
The TI2-C-CLWDA
CLWD condenser
adapter is required.
The T-CHNA High N.A. common
condenser lens unit is required.
TE-C ELWD-S condenser
The ELWD-S condenser is composed of a condenser turret and a dedicated ELWD condenser.
Specifications of the ELWD-S condenser are as follows: NA 0.3, WD 65 mm
Notes on the use of the ELWD-S condenser
When using an ELWD-S condenser, be sure to decrease the light intensity before switching the turret
from the position of PH microscopy to that of BF microscopy. Otherwise, the field of view rapidly becomes
bright.
4.1.3 Combination of Optical Elements
When using a side port of the microscope to perform microscopy with a large field with a field number of
equivalent to or more than 22, use the following combination of illumination attachments.
• TI2-D-PD Pillar for Dia Illumination
• TI2-D-LHLED LED Lamp House for Dia Illumination
Large field-of-view microscopy
• Use of a low-magnification lens might cause the field periphery to be too dark.
• When the C mount is used or a stage-up kit is mounted, the maximum field number is 22 on the side
port of this microscope.
• Depending on the type of the objective, the field periphery might be out of focus.
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