Chapter 3 Usage of Components
71
3.12.3 Selecting Filter Cubes
The fluorescent filter cube (mounted on the turret) consists
of the three types of optical components: the excitation
filter (EX filter), the barrier filter (BA filter), and the dichroic
mirror (DM), that are already attached.
Select the filter cube with the combination of suitable
optical components according to the characteristics of the
specimen and the fluorescence dye.
For details on filter cubes, see “4.4.2 Optical Elements
Required for Epi-FL Microscopy.”
UV-2A
C-FL-C
EX 330-380
DM 400
BA 410
Fluorescent filter cube
Replacement of optical elements
Excitation filters, barrier filters, and dichroic mirrors can be purchased separately.
For details on replacing the optical components, see “7.5.3, Replacing an Excitation Filter, a Barrier Filter,
and a Dichroic Mirror.”
Filter cube for large FOV
The Ti2-series FL turret supports the filter cubes for large
FOV that have the following characteristics:
• Supporting a large field: This filter cube supports a
filter with an outer diameter of 32 mm (effective
diameter of 29 mm), and improves the uniformity of the
field of view when a camera with a field number of 22
mm on the imaging surface is used. (Conventional filter
cube uses a 25 mm filter.)
• Applicable microscopy: The thick dichroic mirror is
suitable for applications that require high degree of the
dichroic mirror flatness such as TIRF observation.
• Maintaining mounting compatibility: The mount part
for the FL turret is compatible with the conventional 25
mm-diameter series.
Filter cube for large FOV
Replacement of optical elements
Excitation filters, barrier filters, and dichroic mirrors can be purchased separately.
For details on replacing the optical components, see “7.5.3, Replacing an Excitation Filter, a Barrier Filter,
and a Dichroic Mirror.”
C-FL-HQ filter cube
Nikon provides a C-FL-HQ filter cube that does not include
optical elements. Select a desired combination of three
optical elements (barrier filter, excitation filter and dichroic
mirror) and attach each element to the C-FL-HQ filter cube
for use.
C-FL-HQ filter cube
Attachment of optical elements
For details on how to attach optical elements, see
“7.5.4 Attaching Optical Elements to the C-FL-HQ
Filter Cube.”
Barrier filte
Excitation filte
Dichroic
mirror (internal)
Barrier filte
Excitation filte
Dichroic mirror
(internal)
Barrier filter
attachment position
Excitation filte
attachment
position
Dichroic mirror
attachment
position
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