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Nikon ECLIPSE Ti2-U - Troubles in NAMC Microscopy

Nikon ECLIPSE Ti2-U
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Chapter 5 Troubleshooting
112
5.1.7 Troubles in NAMC Microscopy
Problem Check item
There is no contrast in the NAMC
image.
Polarizer
󱹕
Place the polarizer into the optical path. (
3.5.1, 4.6.2)
Nosepiece / objective
󱹕
Place the NAMC objective into the optical path. (
3.9.1, 4.6.2)
Condenser module
󱹕
Place the NAMC module into the optical path (
3.4.6, 4.6.2)
Condenser
󱹕
Use a NAMC condenser lens. (
3.4.1, 4.6.2)
Combination of optical elements
󱹕
Confirm that the combination of the objective and the NAMC module is correct.
(
4.6.3)
Modulator adjustment
󱹕
Adjust the position of the modulator of the objective. (
3.4.8)
The contrast in the NAMC image is
poor.
Aperture diaphragm
󱹕
Fully open the aperture diaphragm. (
3.4.5)
Slit adjustment
󱹕
Adjust the slit of the NAMC module according to the modulator position of the
objective. (
3.4.8)
󱹕
If the specimen is of a drop shape, it might have lens effect, so readjust the slit at
the desired position. (
3.4.8)
NAMC image quality is poor.
Condenser module
󱹕
Check if there is dust or scratches on the plastic optical system of the NAMC
module. (
4.6.2)
Wiping resin optical elements with lens tissue might cause a scratch on the surface.
Wiping with solvent might harm the surface. Use a simple cleaning method, such as
with a blower.
Nosepiece / objective
󱹕
Be careful not to turn the correction collar when turning the modulator ring. (
3.4.8)
If the correction collar turned by mistake, adjust it again. (
3.10.1)

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