Nikon TE2000 User Manual

Turning on the system:
1) Turn on the main switch (A).
2) Turn on the computer (B).
3) If you need the mercury lamp,
turn on the switch (C) and then
press ignition (D).
4) Log onto the computer through
“MIC users” and start the
“NIS-Elements” software.
Turning off the system:
1) If you are the last user of the day, or if there is
more than 30 min interval before the next user is
booked, turn off the mercury lamp (C).
2) Close the software and “turn off” the computer
through the start menu.
3) Turn off the main switch (A).
4) Fill out the logbook.
Nikon TE2000 fluorescent microscope
A
B
C
D

Nikon TE2000 Specifications

General

Type	Inverted Microscope
Optical System	CFI60 infinity optical system
Focus	Coarse and fine focusing
Nosepiece	6-position nosepiece
Eyepiece Tube	Binocular or trinocular eyepiece tubes available.
Compatibility	Compatible with various accessories, including cameras
Stage	Mechanical stage

Summary

System Operation Guide

Turning on the Microscope System

Step-by-step instructions for powering up the Nikon TE2000 fluorescent microscope and its associated hardware components.

Turning off the Microscope System

Procedure for safely shutting down the Nikon TE2000 fluorescent microscope and its components at the end of usage.

Camera Selection and Configuration

Choosing the Right Camera

Guidance on selecting between the Nikon DS-U1 (color) and MuTech MV 1500 (B/W) cameras for microscopy applications.

Microscope Illumination Adjustment

Adjusting Köhler Illumination

Detailed steps to optimize image contrast and uniformity by properly adjusting the Köhler illumination system.

Objective Lens Usage

Objective Lens Specifications and Types

Information on microscope objectives, including NA, WD, phase contrast (Ph), and modulation contrast (MC) markings.

Objective Lens Adjustment

Correction Collar Adjustment for Sharpness

Instructions on adjusting the objective's correction collar to achieve the sharpest image focus, considering sample thickness.

NIS-Elements Software Procedures

Stage and Joystick Connectivity

Connecting the microscope stage and joystick through the NIS-Elements software's 'manage devices' interface.

Multichannel Image Acquisition Setup

Configuring NIS-Elements for acquiring images from multiple fluorochromes or combining fluorescence and DIC modes.

Acquiring Large Images with Automated Stage

Procedure for using the automated stage to scan and stitch multiple images into a larger composite image in NIS-Elements.

Troubleshooting Microscopy Issues

Stage or Joystick Not Responding

Resolving issues where the microscope stage or joystick fails to respond within the NIS-Elements software.

Missing Camera Settings in Software

Troubleshooting steps for when camera settings are not visible or accessible in the NIS-Elements control panel.

Saving Data to the REX Server

Steps for connecting to the REX server to save microscopy data, including necessary credentials and connection procedures.