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OligoMaker+DNA/RNA+Synthesizer+
OligoMaker+ApS!•!Kong!Georgs!Vej!12!•!DK-2000!Frederiksberg!•!Tel:!+45!321!322!00!!
www.oligomaker.com!•!email:!karl@ross.dk! !
9.2 Check Reagents
It is indicated on the display how much reagent is required during synthesis. Make a
visual check on the reagent bottles to verify the amount of reagent which is available.
The numbers can vary and has to be adjusted according to the real use (adjusted on
Synthesis Report, delivered seperately).
On the Opening screen, press ”Prime” on the display. Make sure you have activated the
pressure to be able to prime. Activate the pressure for each reagent and shut down the
pressure to the bottles afterwards. When the pressure is off again, the pressure on P1
and P2 should not fall more than 0,2 during a period of two min. P3 indicates the
pressure on the top gas.
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Manometer
Vac Vacuum when pumping
P1 Pressure on bottles Wash, Deblock, Cap A, Cap B and Oxidizer
P2 Pressure on bottles Activator and Amidites
P3 Pressure on Top gas
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