Olympus BH-2 - User Manual

Phase Contrast on the Olympus BH-2 Microscopes Revision 1 Page 1 of 6
Phase Contrast on the
Olympus BH-2 Microscopes
Revision 1
Copyright © 2021 Carl Hunsinger
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Revision History
Revision
Description of Changes
Date
1
Initial release
May 5, 2021

Olympus BH-2 Specifications

General

Brand	Olympus
Model	BH-2
Category	Microscope
Language	English

Summary

Understanding Phase Contrast Microscopy

Scope of the Document

Outlines the coverage and applicability of the Olympus BH-2 microscope manual.

What is Phase Contrast?

Defines phase contrast microscopy and its use for unstained specimens.

How Does Phase Contrast Work?

Explains the optical principles behind phase contrast imaging.

Phase Contrast Types

Details the two types of Olympus phase contrast optics: Positive and Negative.

Setting Up for Phase Contrast Microscopy

Match Selector Dial with Low-Power Objective

Aligns the condenser dial with the selected low-power phase contrast objective.

Replace Eyepiece with Phase-Centering Telescope

Swaps the standard eyepiece for a phase-centering telescope.

Focus the Phase-Centering Telescope

Adjusts the telescope's focus to clearly view the phase annulus.

Center the Selected Phase Annulus

Aligns the objective's phase ring with the condenser's phase annulus.

Center Remaining Phase Annuli

Repeats the centering procedure for all phase contrast objectives.

Replace Centering Telescope with Eyepiece

Reinstalls the standard eyepiece after centering is complete.

Place Green;Yellow Filter into Filter Receptacle

Installs the specified filter for optimal phase contrast performance.

Using and Maintaining Phase Contrast

Using Phase Contrast

Basic operational steps for phase contrast viewing after setup.

Periodically Verify Centering

Recommends re-checking phase annulus alignment before observation.

About the Phase Contrast Filter

Discusses the importance and impact of the green/yellow filter.

Phase Contrast Artifacts

Explains common visual artifacts like halos and shade-off effects.

How To Contact the Author

Provides contact details for questions or comments regarding the document.