Olympus BX-RFAA - User Manual

Questions and Answers

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Natalie HallAug 15, 2025
Why is the image on my Olympus Microscope low quality?
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  Deborah FosterAug 15, 2025
  If the image observed through your Olympus Microscope appears to be of low quality, not sharp, or has poor contrast, there might be dirt or dust on the objective or filter. You should clean these thoroughly. Also, ensure that the aperture and field iris diaphragms are fully enlarged until they circumscribe the field of view. Finally, verify that the fluorescence mirror unit in use matches the specimen.
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Tony WilliamsonAug 18, 2025
Why is the field of view on my Olympus BX-RFAA Microscope obscured?
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  Christian MorganAug 18, 2025
  If the field of view in your Olympus Microscope is obscured or not evenly illuminated, several factors could be the cause. First, ensure that the objective is correctly engaged in the light path and that the revolving nosepiece clicks correctly into place. Second, verify that the fluorescence mirror unit in use matches the specimen. Third, fully enlarge the field iris diaphragm. Fourth, make sure that the ND slider clicks properly into place. Finally, the mercury burner might not be centered, or the focusing could be defective; in this case, center the mercury burner or perform a focusing adjustment.
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coffeyjamesAug 21, 2025
Why is the mercury burner on my Olympus Microscope flickering?
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  Melissa JenkinsAug 21, 2025
  If the mercury burner in your Olympus Microscope is flickering or appears dark, it could be due to several reasons. If it is soon after ignition, allow it to run for at least 10 minutes. If the burner life has expired, and the hour counter indicates 200 hours (USH102D) or 300 hours (HBO103W/2), replace it. Also, the burner might be deviated from the optical axis, so you should center the mercury burner.
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Terry ShannonAug 25, 2025
How to fix a dark field of view on an Olympus Microscope?
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  carsonmatthewAug 25, 2025
  If the bulb lights up but the field of view remains dark on your Olympus Microscope, ensure that the shutter is released. Also, check if the ND filter is engaged in the light path and disengage it if necessary. Verify that the fluorescence mirror unit is correctly engaged in the light path and that the aperture and field iris diaphragms are fully enlarged until they circumscribe the field of view. Finally, confirm that the fluorescence mirror unit in use matches the specimen.
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rebekahcardenasAug 27, 2025
What to do if the Olympus BX-RFAA turns on but the mercury burner won't light?
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  marylongAug 27, 2025
  If the main switch of your Olympus Microscope turns the system on, but the mercury burner does not light, check that the connectors are firmly connected. Ensure the mercury burner is attached. Verify that the safety device in the lamp housing is correctly set up. If auto ignition is malfunctioning, set the main switch of the power supply unit to “ ” (OFF) then “ I ” (ON) again; repeat if necessary.
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Kelli HerreraAug 30, 2025
Why does the field of my Olympus BX-RFAA Microscope have dark spots?
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  gerald79Aug 30, 2025
  If the field of your Olympus Microscope contains dark, spot-like areas, this may be caused by dirt or dust on the burner or on the burner side of the collector lens. Clean these components.
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Jeffrey BrandtSep 2, 2025
Why don't the motorized controls on my Olympus BX-RFAA work?
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  Travis DrakeSep 2, 2025
  If the motorized controls on your Olympus Microscope are not functioning, it is possible that the connectors are not correctly connected. Ensure that all connectors are firmly connected.
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Jeffrey EsparzaSep 5, 2025
Why won't the main switch turn on my Olympus BX-RFAA?
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  harperkatrinaSep 5, 2025
  If the main switch of your Olympus Microscope cannot turn the system ON, ensure that the power cord is connected firmly.
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Vanessa HarrisonSep 8, 2025
Why won't the motorized revolving nosepiece rotate on my Olympus BX-RFAA Microscope?
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  michaelfloresSep 8, 2025
  If the motorized revolving nosepiece of your Olympus Microscope will not rotate, the motorized revolving nosepiece connector may not be correctly connected. Re-attach the motorized nosepiece by inserting it all the way until it stops.
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Sarah MahoneySep 12, 2025
Why won't the turret rotate on my Olympus Microscope?
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  cynthiaestradaSep 12, 2025
  If the turret on your Olympus Microscope will not rotate, or stops in the middle of movement, it may be because the fluorescence mirror unit is slipping out of the turret. Remove the turret and place the fluorescence mirror unit in the correct position securely.

Summary

SAFETY PRECAUTIONS

Safety Symbols

Explains symbols for high voltage, hot surfaces, and manual reading to ensure safe operation.

USING THE CONTROLS

1 General Precautions for Observation

Provides essential precautions for safe and effective observation setup and usage.

2 Selecting the Fluorescence Mirror Units

Guides users on choosing correct fluorescence mirror units based on excitation wavelength and specimen requirements.

3 Objectives for Various Observation Modes

4 Turning the Power Supply Unit On

Step-by-step instructions for safely powering on the mercury lamp power supply unit.

7 Centering the Mercury Burner

Detailed steps for aligning the mercury burner for uniform and bright illumination.

8 Using the ND Filters

SIMULTANEOUS FLUORESCENCE OBSERVATION METHODS

TROUBLESHOOTING GUIDE

ASSEMBLY

9-2 Detailed Assembly Procedures

Olympus BX-RFAA Specifications

General

Excitation Turret	6-position turret
Optical System	Infinity Optical System
Magnification Range	Dependent on objective lenses used
Stage	Not applicable
Illumination	Fluorescence
Light Source	Mercury or Xenon lamp
Filter Cubes	Up to 6 positions
Nosepiece	Not applicable
Focus Drive	Not applicable
Camera Port	C-mount
Compatible Microscopes	BX series microscopes