Opti-Sciences, Inc. CCM-200 plus operator's manual
How it Works
Chlorophyll has several distinct optical absorbance characteristics that the CCM-200 plus
exploits in order to determine relative chlorophyll concentration. Strong absorbance bands are
present in the blue and red but not in the green or infrared bands.
Figure 1 chlorophyll absorbance
CCI = %Transmittance at 931nm. / %Transmittance at 653nm.
The CCM-200 plus uses transmittance to estimate the chlorophyll content in leaf tissue. Two
wavelengths are used for absorbance determinations. One wavelength falls within the
chlorophyll absorbance range while the other serves to compensate for mechanical differences
such as tissue thickness (see figure 2). The meter measures the transmittance of both
wavelengths and calculates a CCI (chlorophyll content index) value that is proportional to the
amount of chlorophyll in the sample.
Note: CCI value is a relative chlorophyll value. Absolute chlorophyll content per unit area is not
computed. CCM-200 plus measurements however can be correlated to ground/solvent analysis.
Figure 2. LED Spectrum
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