Partec CyFlow space - Default Chapter; Table of Contents

Introduction to CyFlow
Rev. 007 Date 2010-01-13 CyFlow
®
space Instrument Operating Manual 2/26
Table of contents
Introduction to CyFlow
®
space ....................................................................................................................... 3
Typical Steps of Particle Analysis .................................................................................................................. 4
Operating Basics .............................................................................................................................................. 5
Switching on the CyFlow
®
space ................................................................................................................. 5
Multi laser Measurements .............................................................................................................................. 6
Starting a Measurement ................................................................................................................................. 7
Switching off the CyFlow
®
space ................................................................................................................. 8
True Volumetric Absolute Counting – Overview ............................................................................................ 9
Performing True Volumetric Absolute Counting ........................................................................................... 10
Optical Standard Setup – The Parameters .................................................................................................. 11
Optical Flow Geometry ................................................................................................................................. 12
Instrument Settings ....................................................................................................................................... 12
The Parameter Setup Dialog Box: Pulse Height, Area and Width ............................................................... 13
Trigger .......................................................................................................................................................... 14
PMT High Voltage, Gain, and Log Amplification .......................................................................................... 15
Sample Speed .............................................................................................................................................. 16
Threshold: Lower Level (L-L) ....................................................................................................................... 17
Appendix ......................................................................................................................................................... 18
Installation Requirements ............................................................................................................................. 18
Instrument Setup – Overview ....................................................................................................................... 19
Instrument Setup – Step by Step ................................................................................................................. 20
The Flow Cuvette ......................................................................................................................................... 21
Maintenance and Service ............................................................................................................................. 22
Transport and Storage.................................................................................................................................. 22
Disposal ........................................................................................................................................................ 22
Laser Safety ................................................................................................................................................. 23
CyFlow
®
space - Technical Specifications ................................................................................................... 24
For in vitro diagnostic use with Partec recommended IVD reagents.
The Partec CyFlow
®
space flow cytometer complies with the European IVD Directive 98/79/EC and is
therefore marked with the CE sign.
© 2010 Partec GmbH
Otto-Hahn-Str. 32
D-48161 Münster
Germany
IVD