Operating Basics
Rev. 007 Date 2010-01-13 CyFlow
®
space Instrument Operating Manual 5/26
Operating Basics
Switching on the CyFlow
®
space
Notes
As sheath fluid you may
use optimized Partec
Sheath Fluid or prepare it
yourself.
To prepare sheath fluid,
fill destilled water into a
glass bottle or an
Erlenmeyer flask (refer
to Application Note:
”Sheath fluid pre-
paration”), through a
small size filter mesh
(< 0.2 µm). Degas the
water by connecting it to
vacuum (e.g. a water jet
pump). Place the bottle
onto a heated stirrer for
about 15 minutes:
Remember: The smaller
the particles in your
analysis are, the more
critical clean sheath fluid
becomes!
It is recommended to
replace the sheath fluid
at least once a week or
before any daily use.
For information how to
work with Windows®
please consult the
manual "Introducing
Microsoft Windows" or
books available in your
local book shop.
1. Check SHEATH and WASTE bottle
Make sure SHEATH bottle is filled with no more than
1600 ml of Sheath Fluid and is closed tightly with the
screw top. Tilt sheath bottle in order to release air
bubbles trapped in the yellow in-line filter!
Make sure the WASTE bottle is empty.
2. Switch on the instrument
The CyFlow
®
space Flow Cytometer is operated with 100
- 240 V AC.
Switch on main power at the left side of the instrument.
The 488nm laser has its own power button closeby,
please also switch on the 488 nm laser.
All other lasers are software controlled (for details please
refer to next page)
3. Control of the 488nm laser output power (for 200
mW high power laser only)
The 200 mW high power laser can be adjusted in its
operation power between 50 and 200 mW. For power
selection please doubleclick the icon shown on the left
(488-200)
To change selected laser power please type in:
p=value enter
To inquire about actual selected laser power type in:
?p enter
3. Switch on peripheral devices
Switch on printer.
4. Switch on computer
Finally, switch on computer by Computer Power switch.
After some ten seconds, the display shows the
Windows® desktop.
5. Start instrument operating software
Move mouse pointer (arrow on display) onto FloMax
®
or
other instrument control program icon.
Doubleclick on the icon with left mouse button.
- after a few seconds, the display shows the FloMax
®
Welcome window. By confirming with “OK” the CyFlow
®
space is initialized and the last used instrument settings
are loaded.
The display shows empty histograms.
6. Cleaning
Use speed 4. Run 1.6 ml Partec Cleaning Solution until
sample tube is empty. Activate CLEAN. Run a second
tube with Partec Sheath Fluid for 2 minutes.
Now, the CyFlow
®
space is ready for acquisition.
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