Partec CyFlow space - Starting a Measurement

Operating Basics
Rev. 007 Date 2010-01-13 CyFlow
®
space Instrument Operating Manual 7/26
Starting a Measurement
Notes
See FloMax
®
- Acqui-
sition and Instrument
control manual on how
to change instrument
settings.
When removing a tube
from the sample port,
the BioSafety system is
activated to prevent
dropping and
contamination by
hazardeous material.
Simultaneously, a
cleaning cycle is
performed to avoid
cross-contamination
between individual
samples.
or
or
1. Make sure your flow cytometer is ready for
analysis and the operating software is prepared for
the measurement.
2. Prepare sample according to the Application
Notes and preparation procedures, resp. Use not
less than 830 µl for true volumetric absolute
counting and not more than 2.8 ml. For smaller
amounts of sample use Partec “Small Volume
Sample Tubes” (Product no. 04-2010, however
these tubes are not compatible with the True
Volumetric Absolute Counting option, page 10).
3. Insert sample tube onto the sample port until
you recognize a "click". The sample should be fully
mounted within a second.
Now the measurement (acquisition) starts
automatically – the operating software indicates the
Prerun, Stabilize, Run and Count status.
Instrument status is also highlighted by LEDs on the
front side of the CyFlow
®
space.
In the Prerun phase, cells are quickly transported
to the position of analysis, the flow cuvette.
During the Stabilize phase, the system waits a
given time for the flow to reach slow speed required
for analysis.
In the Run phase, cells are analysed and classified
into histograms on the display in realtime.
In the Count phase, cells are counted for a given
volume. Do not change instrument settings in the
count phase.
After the count phase, the acquisition finishes
automatically. To finish the acquisition before this
manually, click the end icon or end button, or simply
remove the sample tube from the sample port.
The BioSafety cycle is started.
Status changes to Clean and then Ready.
To restart an acquisition without removing the
sample tube, click onto start.
4. To save results, click on the disk icon.