PerkinElmer 200A Series User Manual (English)

Questions and Answers:

D
Douglas PrestonAug 19, 2025
Why is there excessive noise in my PerkinElmer 200A?
- M
  Melinda StricklandSep 12, 2025
  Excessive noise can occur due to several reasons: * The flow cell might be contaminated, requiring cleaning. * The mobile phase may not be sufficiently degassed; ensure thorough degassing. * Impurities in the mobile phase can cause noise; purify or replace it with a higher quality mobile phase. * The gain level of the recorder could be too high; adjust it accordingly. * The pump might be faulty; it is recommended to check the pump.
R
Rick EvansAug 21, 2025
What to do if my PerkinElmer 200A Laboratory Equipment shows 'Xe LAMP ERROR'?
- K
  Kendra MilesAug 21, 2025
  If the 'Xe LAMP ERROR' message appears, follow these steps: 1. Press the CL key to return to the first monitor screen. 2. Wait at least 5 minutes after turning off the lamp to allow it to cool down, then try turning the power on again or relighting the lamp. 3. If the issue persists, replace the lamp with a new one.
C
Charles PerezAug 23, 2025
Why is there excessive drift in my PerkinElmer 200A Laboratory Equipment?
- B
  Brian PadillaAug 25, 2025
  Excessive drift can be caused by several factors: * The instrument may not have warmed up sufficiently; wait at least one hour for it to stabilize. * Mobile phase might be leaking from a fitting; retighten the fitting. * An impurity could be eluting from the column; wait for elution to complete or replace the column. * Significant changes in ambient temperature can cause drift; control the temperature variation. * The mobile phase might not be sufficiently pure; purify or replace it with a higher quality one.
M
Maria WalkerAug 26, 2025
What to do if my PerkinElmer 200A shows 'A/Z OVER RANGE'?
- A
  amy17Aug 26, 2025
  If the 'A/Z OVER RANGE' message appears, follow these steps: 1. Press the CL key to return to the first monitor screen. 2. Change the wavelength. 3. Replace the solvent with another one. 4. Clean the flow cell.
M
Miguel HoustonAug 28, 2025
What to do if my PerkinElmer 200A Laboratory Equipment shows 'DATA BUFFER OVERFLOW'?
- A
  Aaron OneillAug 28, 2025
  If the 'DATA BUFFER OVERFLOW' message appears, follow these steps: 1. Press the CL key to return to the first monitor screen. 2. Check the connection with the interface board.
M
marshallchristopherAug 30, 2025
What to do if my PerkinElmer 200A Laboratory Equipment shows 'SPECTRA ARE MISMATCHED'?
- J
  Jacob WatkinsAug 30, 2025
  If the 'SPECTRA ARE MISMATCHED' message appears, follow these steps: 1. Press the CL key to return to the first monitor screen. 2. Select the correct parameters.
S
Sarah HebertSep 1, 2025
What to do if my PerkinElmer Laboratory Equipment shows 'TIME PROGRAM BUFFER OVER FLOW'?
- D
  Daniel BenderSep 1, 2025
  If the 'TIME PROGRAM BUFFER OVER FLOW' message appears, follow these steps: 1. Press the CL key to return to the first monitor screen. 2. Delete unnecessary steps from the time program.
D
dhansenSep 3, 2025
What to do if my PerkinElmer Laboratory Equipment shows 'NO SPECTRA'?
- H
  hjohnsonSep 3, 2025
  If the 'NO SPECTRA' message appears, follow these steps: 1. Press the CL key to return to the first monitor screen. 2. Enter a number corresponding to an existing spectrum. 3. Perform spectrum acquisition.
J
jstricklandSep 5, 2025
Why does my PerkinElmer 200A have a poor S/N ratio?
- F
  Frank WilliamsSep 5, 2025
  A poor signal-to-noise ratio can be attributed to several factors: * The flow cell may be contaminated; clean the flow cell. * The xenon lamp may be off-position; adjust the lamp position. * There might be an error in flow cell mounting; correctly mount the flow cell. * The flow cell surface could be scratched; replace the flow cell.
A
Arthur RossSep 7, 2025
What to do if my PerkinElmer 200A Laboratory Equipment shows 'Hg LAMP ERROR'?
- M
  Matthew MasonSep 8, 2025
  If the 'Hg LAMP ERROR' message appears, follow these steps: 1. Press the CL key to return to the first monitor screen. 2. Turn the power on again. 3. Replace the Hg lamp with a new one.

PerkinElmer 200A Series Specifications

General

Type	UV/Vis Spectrophotometer
Light Source	Deuterium and Tungsten Halogen lamps
Detector Type	Silicon Photodiode
Model	200A Series
Baseline Flatness	±0.001 Abs
Display	LCD
Interface	RS-232
Power Requirements	100-240 VAC, 50/60 Hz

Summary

Introduction to the Series 200a Fluorescence Detector

About This Manual

Outlines the manual's structure, conventions, and important notes for the user.

Conventions Used in this Manual

Explains text formatting, key notations, and the use of specific terms within the manual.

Notes, Cautions and Warnings

Defines and explains the usage of NOTE, CAUTION, and WARNING terms for highlighting important information.

Warning Signs on the Instrument

Illustrates and explains common warning signs and symbols found on the instrument.

Safety Practices for Instrument Operation

Overview and General Precautions

Introduces safety aspects and details general precautions for instrument use, complementing standard safety codes.

Flammable Chemical Hazards

Warns about ignition and explosion hazards from flammable chemicals and provides precautions.

Physical and Electrical Hazards

Details risks from electric shock, xenon lamp issues, hot parts, instrument weight, and direct lamp viewing.

Solvent Handling and Disposal

Provides guidelines for safe handling, use, and disposal of solvents, including precautions against toxic or corrosive substances.

Operating and Environmental Conditions

Specifies recommended conditions for instrument operation, including temperature, humidity, and atmosphere.

Electrical Safety and Regulatory Compliance

Covers electrical safety practices, grounding requirements, and regulatory compliance information.

Instrument Warning Labels

Illustrates and explains the various warning labels found on the instrument for user safety.

System Description

Overview of the System

Describes the components of the liquid chromatograph system and their interconnections for analysis.

Control Switches and Indicators

Details the front panel switches, indicators, LCD, and UI pad for instrument control and status monitoring.

The Rear Panel

Identifies and explains the connectors and features located on the rear panel of the instrument.

Description of Fluorometry

Explains the principles, advantages, and analysis notes related to fluorometry detection methods.

Principle of Operation

Illustrates and explains the functional block diagram and operational flow of the detector's components.

Features of Light Source

Describes the characteristics and safety features of the instrument's xenon lamp light source.

Specifications

Lists the detailed technical specifications, including optical system, light source, wavelength ranges, and performance metrics.

Installation Guide

Unpacking the Instrument

Provides instructions and warnings for safely unpacking the instrument upon delivery.

Installation Conditions

Specifies the power supply, frequency, and consumption requirements for instrument installation.

Installation Space Requirements

Details the necessary physical space and table load-bearing capacity for instrument installation.

Environmental Conditions for Installation

Outlines the required ambient temperature, humidity, and atmospheric conditions for proper instrument installation.

Assembling the Instrument

Guides the user through the process of removing transport screws and assembling the instrument components.

Tubing Arrangements

Explains how to connect the tubing for the pump, autosampler, and detector for system integration.

Using the UI Pad

Describes how to use the User Interface (UI) pad for operating the instrument and entering parameters.

Instrument Operation Procedures

Turning on the Detector Power

Details the procedure for powering on the instrument and the initial self-diagnosis sequence.

Entering Parameters and Setup

Covers general guidelines for data entry and initial setup procedures like communication channel and LCD contrast.

Adjusting Auto Zero and Wavelength

Describes how to set the auto zero function and configure excitation/emission wavelengths for measurements.

Utility Settings and Functions

Details various utility settings including offset, time constant, PMT voltage, and other operational parameters.

Spectrum Measurement Procedures

Guides on performing spectrum measurements for excitation and emission scans, including data memorization.

Recorder and Output Settings

Explains how to configure recorder settings like range, speed, and spectrum output factors for data logging.

Time Program Operation

Covers setting up, editing, and running time programs for automated measurements, including parameter changes over time.

Shutdown and Flushing Procedures

Details the correct procedure for shutting down the instrument and flushing the flow cell to prevent clogging.

Before Performing Analysis

Lists essential checks and precautions to be taken before starting analytical measurements, focusing on safety.

Maintenance and Inspection Methods

Operational and Performance Checks

Outlines procedures for checking the instrument's operational status and performance metrics like lamp energy and sensitivity.

Wavelength Accuracy Check and Calibration

Details methods for checking and calibrating the wavelength accuracy of the instrument using a Hg lamp.

Leak Sensor Checks and Cleaning

Explains how to check the functionality and clean the instrument's leak sensor to ensure proper operation.

Periodic Maintenance and Inspections

Lists routine checks for lamp status, leakage, sensitivity, and wavelength accuracy at daily, monthly, and yearly intervals.

Flow Cell Maintenance

Covers procedures for checking, washing, disassembling, cleaning, and storing the flow cell unit to maintain performance.

Lamp and Fuse Replacement

Provides instructions for replacing the xenon lamp and fuses, including critical safety precautions.

Troubleshooting Guide

Common Troubleshooting Symptoms

Addresses common issues like initialization failure, non-working keys, excessive noise, poor S/N ratio, and lamp life.

Addressing Instrument Errors and Malfunctions

Details specific error messages, their causes, and remedies, including steps for parameter recovery and contact information.

Appendix Information

Description of Contact Signals

Explains the types and functions of contact signals used for communication between instrument modules.

Glossary of Technical Terms

Provides definitions for key terms and acronyms used throughout the manual for better understanding.

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PerkinElmer 200A Series User Manual

Table of Contents