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PerkinElmer LabChip GX Touch User Manual

PerkinElmer LabChip GX Touch
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User Manual
LabChip
GX Touch/GXII Touch
P/N CLS151164 Rev. B
Publication Date: October 12, 2018

Table of Contents

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PerkinElmer LabChip GX Touch Specifications

General IconGeneral
ApplicationDNA, RNA, and protein analysis
Detection MethodLaser-induced fluorescence
ThroughputUp to 96 samples per run
WeightApproximately 25 kg
Power Requirements100-240 V AC, 50/60 Hz
Operating Temperature15-30°C
ConnectivityUSB, Ethernet
DesignBenchtop
TypeAutomated electrophoresis system
TechnologyMicrofluidics
Sample TypesDNA, RNA
Sample Volume2 µL
Analysis Time30 seconds to 2 minutes per sample
Sensitivity0.1 ng/µL for DNA
Operating Humidity20% to 80% RH, non-condensing

Summary

Preface

Proper Equipment Operation

Contains safety warnings for instrument use.

Instrument Safety

Required Training

Ensures personnel are trained in general and specific safety practices.

Chemical Safety

Guidelines for handling chemicals safely with the instrument.

Laser Safety

Safety precautions related to the instrument's laser system.

Electrical Safety

Precautions for safe use of electrical components.

Mechanical Safety

Safety measures concerning moving parts and pinch hazards.

Introduction

Usage

Information on the intended use of the LabChip GX Touch instruments.

Assay User and Quick Guides

Guides for specific assays, including preparation and troubleshooting.

Principles of Operation

Explanation of how the LabChip GX Touch assay technology works.

Preparing the Instrument to Run Samples

Opening the LabChip GX Touch Software

Instructions for launching the instrument control software.

Purging the Pressure Lines

Procedure to clear debris from pressure lines for optimal performance.

Inserting the Chip

Step-by-step guide for correctly inserting the microfluidic chip.

Calibrating the Instrument to Run Protein Clear HR Assays

Procedure for calibrating the instrument for specific assays.

Priming the Chip Before the Run

Process to prepare the chip for assay analysis.

Loading the Plate, Ladder Tube, and Buffer Tube

Instructions for loading necessary consumables into the instrument.

Running an Assay

Defining the Run Parameters

Configuring settings such as assay type, plate, and wells for a run.

Monitoring the Run

Observing the progress and status of an ongoing assay run.

Editing the Analysis Settings during a Run

Adjusting analysis parameters while an assay is in progress.

Stopping a Run before the Run is Complete

Procedure to manually halt an assay run before it finishes.

Removing the Chip

Procedure for safely unloading the chip after a run.

Exporting Data

Export Examples

Illustrations and formats for exporting different data types.

Selecting the Auto Export Settings

Configuring automatic export of data tables, graphs, or gels.

Exporting a Data File after a Run is Complete

Steps to export data files once the assay run has finished.

Viewing the Data

Selecting the Well Data to View

Choosing specific wells for data visualization and analysis.

Viewing Data in the Egram/Gel Tab

Displaying electropherogram and gel data for selected wells.

Viewing Graphs in the Egram Tab

Visualizing assay results as graphs in the EGram tab.

Zoom In and Zoom Out

Adjusting the magnification of graph and gel views.

Software Reference

Navigation Bar

Overview of the main navigation buttons and their functions.

Home Window

The primary interface for initiating operations like priming, running, and washing.

Run Window

Interface for defining assay run parameters via multiple tabs.

Status Window

Provides real-time status of the instrument, chip, and run operations.

Tools Window

Access point for diagnostics, export settings, optics calibration, and more.

Analysis Settings Tab

Adjusting parameters for peak detection and analysis during runs.

Maintenance and Service

Cleaning the Chip Interface

Daily and monthly procedures for cleaning critical interface components.

Calibrating the Optics

Procedure to calibrate instrument optics for consistent fluorescence readings.

Cleaning the Instrument Electrodes

Daily cleaning of electrodes to ensure proper conductivity.

Changing the Fuses

Procedure for safely replacing instrument fuses.

Running Software IQ

Verifies proper software installation and integrity.

Running the Instrument Diagnostics Tests

Performing tests to verify instrument operation and components.

Troubleshooting and Diagnostics

Error Messages

List of common error messages and their potential causes/solutions.

Device <Name> is Disconnected

Troubleshooting steps for communication loss with connected devices.

Focus Failed

Diagnosing and resolving issues related to the instrument's focus mechanism.

Calibration Failures

Identifying causes and solutions for assay calibration failures.

Software Problems

Addressing common issues like inability to save files or software lock-ups.

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