November 2021
Quick-Start Protocol
EZ1&2™ ccfDNA Kit
The EZ1&2 ccfDNA Kit (cat. no. 954854) is shipped at room temperature (15–25°C). Upon
receipt, store all kit components dry at room temperature.
Further information
EZ1&2 ccfDNA Kit Handbook
: www.qiagen.com/HB-2915
Safety Data Sheets: www.qiagen.com/safety
Technical assistance: www.support.qiagen.com
Notes before starting
Plasma samples should be at room temperature before use. If plasma/serum was stored
frozen, thaw the samples by incubating at 37°C in a water bath.
If the sample volume is less than 2 ml, 4 ml, or 8 ml, adjust the volume with PBS to 2 ml,
4 ml, or 8 ml.
Procedure
1. Load reagent cartridges into the cartridge rack.
2. Resuspend Magnetic Bead Suspension EZ by vortexing briefly and then tap to ensure all
the liquid is at the bottom of the tube. Remove the cap from the tube with the bead
solution placed in position 11 of the EZ1&2 ccfDNA cartridge (see Figure 1).
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