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Qiagen TissueLyser II - User Manual

Qiagen TissueLyser II
48 pages
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May 2016
TissueLyser II
User Manual
For disruption of up to 48 or 192 biological
samples

Table of Contents

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Summary

Safety Information

Proper Use

Details correct and safe usage, user qualifications, and approved accessories for the TissueLyser II.

Electrical Safety

Covers electrical hazards, lethal voltages, and safety precautions for operating the TissueLyser II safely.

Environment and Operating Conditions

Specifies environmental factors like explosive atmospheres, operating temperature, and humidity for safe operation.

Biological Safety

Addresses handling of potentially infectious samples and outlines safe laboratory procedures.

Chemicals and Toxic Fumes

Highlights hazards of chemicals and toxic fumes, requiring appropriate protective measures and ventilation.

Mechanical Hazards

Warns about moving parts and potential damage or injury if components are not securely attached.

Waste Disposal

Provides guidance on the proper disposal of used labware and waste according to local safety regulations.

Introduction

About this User Manual

Outlines the structure of the manual and the topics covered in each of its sections.

Intended Use of the TissueLyser II

Defines the primary applications for the TissueLyser II, focusing on molecular biology processes.

Damage to Clamps

Explains how improper adapter use can damage TissueLyser II clamps and recommends approved accessories.

User Requirements

Details the general competence and training needed for users to operate the TissueLyser II.

General Description

TissueLyser II Principle

Explains the bead-milling technology used for sample disruption and homogenization by high-speed shaking.

Features of the TissueLyser II System

TissueLyser II Features

Describes the main features, oscillation frequency, clamps, and safety mechanism of the TissueLyser II.

TissueLyser Adapter Set 2 x 24

Description

Details the Adapter Set 2 x 24, its capacity for 48 samples, and cleaning instructions.

TissueLyser Adapter Set 2 x 96

Description

Details the Adapter Set 2 x 96, its capacity for 192 samples, and cleaning instructions.

Grinding Jar Set, S. Steel

Description

Describes the stainless steel grinding jar set, its capacity for 2 samples, and disruption conditions.

Grinding Jar Set, Teflon

Description

Describes the Teflon grinding jar set, its capacity for 2 samples, and disruption conditions.

TissueLyser Single-Bead Dispensers

Description

Details the single-bead dispensers, their sizes, and their capacity for dispensing beads into sample containers.

TissueLyser Bead Dispensers, 96-Well

Description

Details the 96-well bead dispensers, their sizes, and how they dispense beads into racks of collection microtubes.

Beads

Available Beads

Lists the available beads for the TissueLyser II by material, size, and catalog number.

Applications

Sample Types

Outlines the types of biological samples that can be disrupted and homogenized using the TissueLyser II.

Installation Procedures

Unpacking the TissueLyser II

Provides step-by-step instructions for unpacking the TissueLyser II and verifying its supplied contents.

Transport Security Device Removal

Guides on how to remove the transport security device screws before operating the instrument.

Site Requirements

Specifies the environmental and location requirements for installing and operating the TissueLyser II.

Power and Grounding Requirements

Covers power supply specifications, voltage regulation, surge protection, and proper grounding for the instrument.

Transporting and Repacking

Details how to safely transport and repack the TissueLyser II, emphasizing retaining original packaging.

Installation of Adapter;Jar Sets

Attaching Adapter;Jar Sets

Provides a step-by-step guide on how to securely install adapter sets or grinding jars into the TissueLyser II clamps.

Replacing the Fuses

Procedure

Outlines the procedure for replacing the fuses in the TissueLyser II, including the required fuse type and location.

Operating Procedures

Sample Disruption

Guides users through the step-by-step process of disrupting samples using the TissueLyser II's controls.

Storing Disruption Parameters

Explains how to save and store up to nine disruption parameter sets for different applications.

Displaying Operating Info

Details how to view the instrument's accumulated operating time and its current software version.

Maintenance Procedures

Servicing and Warranty

Provides information regarding the warranty period and the scope of servicing for the TissueLyser II.

Regular Maintenance

Describes routine cleaning and maintenance tasks required to ensure the reliable operation of the instrument.

Cleaning Agents

Lists recommended disinfectants and detergents for cleaning the TissueLyser II and its accessories.

General Cleaning Instructions

Provides general guidelines and precautions for cleaning the TissueLyser II and its components safely.

Regular Safety Checks

Outlines periodic checks for the limit switch and brake to ensure correct operation and safety.

Troubleshooting Guide

Error Codes

Lists common error codes displayed by the TissueLyser II and provides suggested solutions and troubleshooting steps.

Appendix A

Technical Data

Presents detailed technical specifications of the TissueLyser II, including power, dimensions, and environmental conditions.

WEEE Disposal

Provides information on the proper disposal of the TissueLyser II as electronic waste according to regulations.

Declaration of Conformity

Company Information

States the company name and address and how to request the Declaration of Conformity.

Appendix B

Liability Clause

Details the terms and conditions of the warranty, including limitations and exclusions for repairs.

Notes

General Notes

Provides space for user notes and remarks related to the operation or maintenance of the instrument.

Qiagen TissueLyser II Specifications

General IconGeneral
Power Supply100-240 V, 50/60 Hz
TimerYes
Speed3-30 Hz (180-1800 rpm)
Noise Level<70 dB
ApplicationsTissue disruption, homogenization, and cell lysis

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