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Qiagen EZ1 Advanced - User Manual

Qiagen EZ1 Advanced
126 pages
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May 2008
Sample & Assay Technologies
EZ1
®
Advanced User Manual
9001410, 9001411
1050261
QIAGEN GmbH, D-40724 Hilden
R2

Table of Contents

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Summary

Safety Information

1.1 Proper Use

Guidelines for correct and safe operation of the EZ1 Advanced.

1.2 Electrical Safety

Information on electrical hazards and safe connection procedures.

1.3 & 1.4 Environmental and Biological Safety

Precautions for operating conditions and handling biological agents.

1.5 & 1.6 Chemicals and Waste Disposal

Safety measures for hazardous chemicals and proper waste disposal.

1.7, 1.8 & 1.9 Hazard Awareness

Information on mechanical, heat, and UV radiation hazards.

1.10 Symbols on EZ1 Advanced

Explanation of safety symbols used on the instrument.

1.11 Additional Symbols in User Manual

Explanation of symbols used within the user manual.

Introduction

2.1 About This User Manual

Overview of the manual's content and structure.

2.2 General Information

Details on technical assistance, policy, and version management.

2.3 Intended Use of EZ1 Advanced

Description of the instrument's purpose and user requirements.

General Description

3.1 External Features of EZ1 Advanced

Description of the instrument's physical components and interfaces.

3.2 Internal Features of EZ1 Advanced

Overview of the instrument's internal mechanisms and systems.

Installation Procedures

4.1 Requirements

Site and environmental requirements for instrument installation.

4.2 AC Power Connection

Guidance on connecting the instrument to the power supply.

4.3 Hardware Installation

Steps for unpacking and installing the EZ1 Advanced instrument.

4.4 PC Software Installation

Instructions for installing the EZ1 Advanced Communicator software.

4.5 Multiple Instrument Installation

Procedures for connecting multiple instruments to a single PC.

4.6 Transportation and Removal

Guidelines for safely moving and removing the instrument.

General Operation

5.1 Overview

Step-by-step guide to operating the EZ1 Advanced instrument.

5.2 EZ1 Advanced Card Operations

Instructions for inserting and removing the EZ1 Advanced Card.

5.3 & 5.4 Powering and Door Operation

Procedures for switching the instrument on/off and operating the door.

5.5 Protocol Run Management

How to start, finish, and stop protocol runs.

5.6 Worktable Setup Procedures

Guidance on setting up the worktable, including loading components.

5.7 UV Lamp Usage

Information on operating and managing the UV lamp for decontamination.

5.8 Manual Operation Modes

Description of different manual operation functions.

5.9 Instrument Testing

Procedures for testing instrument functions like axis, temp, serial port, and version.

5.10 System Setup

Configuration options for date, time, serial port, and maintenance reminders.

5.11 Bar Code Reader Usage

How to use the bar code reader to scan and input data.

5.12 External Printer Usage

Instructions for using an optional external printer for report output.

5.13 Report File Generation

Details on creating and managing report files.

5.14 EZ1 Advanced Communicator Software

Using the software for data transfer and management.

5.15 Multiple Instrument Operation

Connecting and operating multiple EZ1 Advanced instruments.

5.16 Pipetting Accuracy Evaluation

Protocols for testing pipetting accuracy and temperature.

Preventive Maintenance

6.1 Regular Maintenance Procedure

Steps for performing regular maintenance after each run.

6.2 Daily Maintenance Procedure

Tasks for daily cleaning and upkeep of the instrument.

6.3 Weekly Maintenance Procedure

Procedures for weekly maintenance, including greasing O-rings.

6.4 Reagents for Decontamination

Recommended reagents for disinfecting the instrument surfaces.

Troubleshooting

7.1 Error Detection and Handling

Identifying and resolving common instrument errors and door issues.

7.2 Error Codes

A list and description of error codes and their meanings.

Glossary

Appendix A

Technical Data

Specifications for power, fuses, and environmental conditions.

Environmental and Transportation Conditions

Details on operating, transport, and storage environmental parameters.

Waste Electrical and Electronic Equipment (WEEE)

Information on the disposal of electronic waste according to EU directives.

EC Declaration of Conformity

Statement of compliance with European directives for the product.

Appendix B

Liability Clause

Details regarding warranty terms and conditions for repairs and modifications.

Qiagen EZ1 Advanced Specifications

General IconGeneral
User InterfaceTouch screen
Power Supply100-240 V, 50-60 Hz
ConnectivityEthernet
Sample TypesSerum, plasma, buffy coat, cells, tissues
Sample Volume200 µL to 1 mL (varies by sample type)
TechnologyMagnetic-particle technology
Nucleic Acid TypeDNA, RNA
Operating Temperature15-30°C
Relative Humidity20–80% noncondensing
Elution Volume25-100 µL
Processing Time20-40 minutes (depending on sample type and protocol)
AutomationFully automated

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