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Qiagen QIAcube - User Manual

Qiagen QIAcube
162 pages
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Version 1.1 June 2008
Sample & Assay Technologies
QIAcube
®
User Manual

Table of Contents

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Summary

Safety Information

Proper Use

Guidelines for correct operation and avoiding hazards.

Electrical Safety

Precautions related to electrical power and connections.

Environment

Recommended operating conditions and environmental considerations.

Biological Safety

Handling of biological samples and infectious agents.

Chemicals

Safety measures when handling chemicals used with the instrument.

Waste Disposal

Procedures for safe collection and disposal of waste.

Mechanical Hazards

Warnings and precautions regarding moving parts and mechanical risks.

Heat Hazard

Information on potential heat-related risks from instrument components.

Translations of Warnings and Cautions

Glossary of safety warning terms in multiple languages.

Symbols on the QIAcube

Explanation of safety symbols and their meanings.

Introduction

About this User Manual

Overview of the manual's structure and content.

General Information

Details on technical assistance, policy, and version management.

Intended Use of the QIAcube

Description of the instrument's purpose and target users.

General Description

QIAcube Principle

Explanation of the automated sample preparation workflow.

External Features of the QIAcube

Identification and description of the instrument's external components.

Internal Features of the QIAcube

Identification and description of the instrument's internal components.

Installation Procedures

Requirements

Site, power, and environmental prerequisites for installation.

AC Power Connection

Instructions for connecting the instrument to electrical power.

Unpacking the QIAcube

Steps for safely removing the instrument from its packaging.

Installing the QIAcube

Procedures for setting up the instrument after unpacking.

Packing the QIAcube

Instructions for safely packaging the instrument for transport.

Operating Procedures

QIAcube Software

Description of the software interface and button functions.

Running a QIAGEN Protocol

Step-by-step guide for starting and executing protocols.

Setting up the Worktable

Instructions for loading reagent bottles, tip racks, and samples.

Installing and Deleting Protocols

Procedures for managing protocols on the instrument.

Saving Data Files

How to save run reports, log files, and other data.

General Information

Accessing system information and software versions.

Operating the Shaker

How to use the shaker function independently.

Operating the Centrifuge

How to use the centrifuge function independently.

Maintenance Procedures

Cleaning Agents

Recommended disinfectants and detergents for cleaning.

Disinfection

Procedures for disinfecting instrument surfaces and components.

Removal of RNase Contamination

Steps for removing RNase contamination.

Removal of Nucleic Acid Contamination

Steps for removing nucleic acid contamination.

Regular Maintenance Procedure

Routine tasks after each protocol run.

Daily Maintenance Procedure

Tasks to perform at the end of each day.

Monthly Maintenance Procedure

Tasks to perform on a monthly basis.

Periodic Maintenance Procedure

Tasks performed as needed or at least every six months.

Cleaning the QIAcube Centrifuge After a Plastic Crash

Specific procedures for cleaning after a plastic crash event.

Troubleshooting

General Information

Information needed to report an error effectively.

Resolving Error Messages and Warnings

Guide to identifying and resolving instrument errors.

Glossary

Appendix A

Technical Data

Specifications and performance details of the instrument.

Environmental Conditions

Operating and storage conditions for the QIAcube.

Storage Conditions

Recommended conditions for storing the instrument.

Mechanical Data and Hardware Features

Physical dimensions and key hardware specifications.

Waste Electrical and Electronic Equipment (WEEE)

Information on proper disposal of electronic waste.

FCC Declaration

Regulatory compliance statement regarding FCC regulations.

EC Declaration of Conformity

Statement of compliance with European directives.

Appendix B

Loading the Centrifuge and Shaker

Diagrams showing correct sample loading for the centrifuge and shaker.

Appendix C

QIAcube Accessories

List of available accessories and their catalog numbers.

Appendix D

Liability Clause

Legal terms and conditions regarding warranty and liability.

Index

Qiagen QIAcube Specifications

General IconGeneral
AutomationFully automated
InterfaceTouchscreen
Elution Volume50-200 µl
Processing Time30-60 minutes per run
Operating Temperature15-30°C
Humidity Range20-80% non-condensing
Sample TypeDNA, RNA, protein
Capacity12 samples per run
Power Supply100-240 V AC, 50-60 Hz
CompatibilityQIAGEN spin-column kits
ThroughputUp to 12 samples per run

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