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Qiagen QIAgility - User Manual

Qiagen QIAgility
334 pages
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Sample & Assay Technologies
Sample & Assay Technologies
1069303 11/2011
Trademarks
QIAGEN
®
, QIAgility
®
, QIAxcel
®
, Rotor-Gene
®
(QIAGEN Group); DECON-QUAT
®
(Veltek Associates Inc.); Gigasept
®
, Mikrozid
®
, Lysetol
®
(Schülke & Mayr GmbH); Microcide SQ
(Global
Biotechnologies, Inc.); Excel
®
, Microsoft
®
, Windows
®
(Microsoft Corporation). Registered names, trademarks, etc. used in this document, even when not specifically marked as such, are not to
be considered unprotected by law.
© 2009-2011 QIAGEN, all rights reserved.
QIAgility
®
User Manual
QIAgility
®
User Manual
November 2011
www.qiagen.com
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Table of Contents

Question and Answer IconNeed help?

Do you have a question about the Qiagen QIAgility and is the answer not in the manual?

Summary

Safety Information

Proper Use

Information on the correct and safe operation of the QIAgility instrument.

Electrical safety

Guidelines for safe handling and connection of the QIAgility to electrical power sources.

Biological safety

Precautions for handling potentially infectious specimens and reagents.

Chemicals

Procedures and precautions for working with hazardous chemicals.

Mechanical Hazards

Safety precautions related to the instrument's moving parts and robotic arm.

Maintenance safety

Safety guidelines to follow during instrument maintenance procedures.

Introduction

General Description

QIAgility principle

Explanation of how the QIAgility automates PCR setup and liquid handling.

Installation Procedures

Instrument delivery and installation

Information on items delivered and initial installation steps.

Computer setup and installation of software

Instructions for setting up the computer and installing QIAgility software.

Software installation

Detailed steps for installing the QIAgility software from CD.

QIAgility Software

Starting the software

Instructions on how to start the QIAgility software.

User management

Features for defining access rights and managing users.

Manage calibration data

Options for transferring and managing instrument calibration settings.

Configure run settings

Options for configuring various pipetting and run parameters.

Set tip usage options

Configuring tip reuse and maximum reuse times for efficiency.

Configure multi-eject options

Setting options for multi-eject functionality during pipetting.

Define worktable setup

Configuration of plates and their functions on the instrument worktable.

Setting tip availability

Setting the availability status of tips for accurate tip picking.

View and edit information using the right-hand pane

Using the right-hand pane to view and edit plate function information.

Programming run files

Procedures for creating and programming run files using wizards or empty projects.

PCR Setup Wizard

Step-by-step guide for setting up PCR reactions using the wizard.

Optimisation Wizard

Guide for setting up optimized reaction matrices with varying reagent concentrations.

Operating Procedures

Starting the software

Instructions for starting the QIAgility software and instrument.

Executing a run

Comprehensive guide to executing a run on the QIAgility.

Q Protocols

Information on using pre-developed Q Protocols for specific applications.

Preparing the worktable

Steps for preparing the worktable with reagents, samples, and plates.

Starting a run

Detailed steps for initiating and starting an instrument run.

Pausing;aborting a run

How to pause or abort an ongoing run.

Pre-run report

Details on generating and reviewing the pre-run report.

Post-run report

Information contained within the post-run report after a run completes.

Importing and exporting reaction;sample data

Methods for importing and exporting reaction and sample data.

Shutting down the software and the instrument

Step-by-step guide for safely shutting down the software and instrument.

Instrument Setup and Calibration

Setup tip ejector

Procedure for calibrating the tip ejector mechanism.

Calibrate plate positions

Steps for calibrating the position of plates on the worktable.

Calibrate plate heights

Procedures for calibrating the vertical position of plates.

Calibrate tip offsets

Calibrating tip offsets to ensure accurate height for different tip types.

Calibrate volumes

Procedures for calibrating the instrument's volume dispensing accuracy.

Maintenance Procedures

Regular maintenance

Procedures for routine maintenance of the QIAgility.

Cleaning the QIAgility

Guidelines for cleaning the instrument surfaces and lid.

Periodic maintenance

Procedures for periodic decontamination and upkeep.

Decontaminating the QIAgility

Steps to decontaminate the worktable to eliminate cross-contamination.

Troubleshooting

System setup

Common issues related to system setup and initial configuration.

Operation

Solutions for common operational problems encountered during runs.

Warnings and errors

Understanding and addressing warnings and errors displayed by the software.

Warning and error messages

Explanation of warning symbols and messages in the software.

Instrument error codes

List and interpretation of instrument-specific error codes.

Self diagnostic tools

Using diagnostic tools to identify and resolve instrument issues.

Mechanical tests

Procedures for performing mechanical tests on the instrument.

Electrical tests

Procedures for performing electrical and communication tests.

Appendix C

Safety Information (French, FR)

Safety information presented in French.

1 Consignes de sécurité

General safety instructions in French for using the QIAgility.

1.1 Utilisation appropriée

French safety guidelines for proper use of the QIAgility.

1.2 Sécurité électrique

French safety information regarding electrical hazards.

1.3 Sécurité biologique

French safety information regarding biological hazards.

1.4 Produits chimiques

French safety information regarding hazardous chemicals.

1.6 Dangers mécaniques

French safety information regarding mechanical hazards.

1.7 Sécurité relative à la maintenance

French safety guidelines for instrument maintenance.

Appendix D

Safety Information (German, DE)

Safety information presented in German.

1 Sicherheitshinweise

General safety instructions in German for using the QIAgility.

1.1 Sachgemäße Handhabung

German safety guidelines for proper handling of the QIAgility.

1.2 Elektrische Sicherheit

German safety information regarding electrical hazards.

1.3 Biologische Sicherheit

German safety information regarding biological hazards.

1.4 Chemikalien

German safety information regarding hazardous chemicals.

1.6 Gefahren durch mechanische Teile

German safety information regarding mechanical hazards.

1.7 Sicherheitshinweise - Wartungsarbeiten

German safety guidelines for instrument maintenance.

Appendix H

Packing the QIAgility

Instructions for packing the QIAgility instrument for transport.

Qiagen QIAgility Specifications

General IconGeneral
TypeAutomated liquid handling system
ManufacturerQiagen
ModelQIAgility
ThroughputUp to 96 samples
Power Requirements100-240 V, 50/60 Hz
SoftwareQIAgility Software
Tip typeDisposable tips
ApplicationsPCR setup
Volume Range1 µl to 1, 000 µl
PrecisionCV < 5%
Typical run timeVaries depending on the protocol
Number of Channels8 channels

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