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XCell ATF Series
REPLIGEN XCell ATF Series User Manual
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XCell
TM
ATF System with C410:V4B Controller
User Guide
9
Figure 4b. Exploded View of
XCell™ ATF 10
Single-use Device with 0.2u PES Hollow Fiber Filter
12
14
Table of Contents
Default Chapter
3
Table of Contents
3
1 Description of the Xcell™ ATF (Alternating Tangential Flow) System and Process
5
Xcell TM ATF System Pump Cycle
7
Xcell™ ATF System Control Scope and Objectives
9
2 Utility Requirements
9
3 Dimensions and Weight
10
4 Xcell™ ATF Pump Housing Assemblies
11
See Appendix 7 for List of Spares and Accessories
16
5 Xcell TM C410V4B Controller Layout
21
General Layout
21
Filtration Assembly (Stainless Steel)
22
Pneumatics Box (P-Box) Components
22
Electrical Box (E-Box)
22
Power Box (Power Separation) Components
24
Primary Pneumatic Services
24
Primary Electric Services
25
Signal
25
6 C410V4B Controller Process and Control
26
Control Overview
26
Control Functional Algorithms
28
Interface and Screens
29
Startup
54
Examples
56
7 Xcell™ ATF Hollow Fiber Module and Diaphragm Replacement
58
Separating the Filtration Assembly from the Bioreactor
58
HFM Removal
58
HFM Insertion
59
Screen Module Replacement
60
Diaphragm Replacement
60
Assembly
61
Use
61
8 Sterilization
62
Preparation of Filtration Assembly for Autoclaving
62
Autoclave Cycle
63
Sterilization of Filtrate / Harvest Line
64
9 Connection to Bioreactor (ATF-To-Bioreactor)
65
Hard Connection
65
10 Maintenance
66
Diaphragm
66
Pump Air Inlet Filter
66
O" Rings, Gaskets and Quick Connects
66
Appendix 1: Cycle Time Vs. Flow Rate
67
Appendix 2: Access Levels to the C410V4B Controller
68
Appendix 3: Controller Lists: Alarm, Warning, Inputs & Outputs
75
Appendix 4: Profinet Communication
77
Appendix 5: Profibus Communication
79
Appendix 6: Delta V Example Configuration
100
Appendix 7: OPC Communication
107
Appendix 8: Audit Trail (if Equipped)
117
Appendix 9: EU Declaration of Conformity
120
Appendix 10: UL Listing
123
Appendix 11: Spares List
124
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REPLIGEN XCell ATF Series Specifications
General
Brand
REPLIGEN
Model
XCell ATF Series
Category
Laboratory Equipment
Language
English
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