REPLIGEN XCell ATF10 User Manual

XCell
TM
ATF System with C410:V4B Controller User Guide
79
d. Set InputPage3.P2_P2_Capture = 1 until the OutputPage4.P2_Cal_Enable bit is
received.
i. Wait for the “RequestedDataPage” to be equal to 4; since cycling through
output data pages is recommended.
ii. Examine OutputPage4.P2_ P2_Capture. When true…
e. Reset InputPage3.P2_P2_Capture = 0; the maximum capture bit is momentarily
asserted.
6) Assert accept bit:
a. While “SendingDataPage” is still equal to 3…
b. Set InputPage3.P2_Accept = 1 until the OutputPage4.P2_Accept bit is received.
i. Wait for the “RequestedDataPage” to be equal to 4; since cycling through
output data pages is recommended.
ii. Examine OutputPage4.P2_Accept. When true…
c. Reset InputPage3.P2_Accept = 0.
d. Controller performs the linear scaling calculation and resets the controller’s
internal calibration enable bit.
7) Clean up: After all calibration activities are complete, be sure to perform the following:
a. Reset parameters that were set during the calibration process.
b. Set “SendingDataPage” = 0 to prepare for next ATF run commands.

REPLIGEN XCell ATF10 Specifications

General

Brand	REPLIGEN
Model	XCell ATF10
Category	Laboratory Equipment
Language	English

Summary

XCell™ ATF System

Description of the XCell™ ATF System and Process

XCell™ ATF System Pump Cycle

Details the operational cycle of the XCell™ ATF system pump.

XCell™ ATF System Control Scope and Objectives

Outlines the control capabilities and goals of the XCell™ ATF system.

XCell™ C410v4B Controller Layout

Filtration Assembly (Stainless Steel)

Details the stainless steel filtration assembly components and connections.

Pneumatics Box (P-Box) Components

Lists and describes the components within the Pneumatics Box (P-Box).

Electrical Box (E-Box)

Details the components housed within the Electrical Box (E-Box).

Power Box (Power Separation) Components

Describes the components located in the Power Box for power separation.

Primary Pneumatic Services

Explains the primary pneumatic connections and services for the system.

Primary Electric Services

Details the primary electrical connections and services required for operation.

C410v4B Controller Process and Control

Control Overview

Provides a general overview of the process and control mechanisms.

Control Functional Algorithms

Explains the algorithms used by the controller for process management.

Interface and Screens

Describes the user interface and various screens of the controller.

Startup

Guides users through the initial setup and startup procedures for the system.

XCell™ ATF Hollow Fiber Module and Diaphragm Replacement

Sterilization

Preparation of Filtration Assembly for Autoclaving

Steps for preparing the filtration assembly before autoclaving.

Autoclave Cycle

Details the recommended autoclave cycle for sterilization.

Connection to Bioreactor (ATF-to-Bioreactor)

Hard Connection

Describes the procedure for making a hard connection to the bioreactor, including SIP.

Maintenance

Diaphragm

Recommendations for diaphragm usage, orientation, and lifespan.

Appendix 2: Access levels to the C410v4B controller

Operators

Engineers

Administrators

Appendix 4: Profinet Communication

Profienet® Infrastructure

Configuration

Appendix 5: Profibus Communication

Remote Control Mode

Input Signals (from Master to XCell™ ATF)

Output Signals (to Master from XCell™ ATF)

OPC Configuration for DCS Integration

Black-Box Configuration

Prerequisites

Appendix 6: Delta V Example configuration

Install the GSD File in Delta V Explorer

Add new Profibus Device

Select Profibus Device Type

Address Setting

Confirm and Select “OK” button

Verify “Enabled”

Create Slot

Appendix 7: OPC Communication

XCell™ ATF Operations- Remote Monitoring and Data Acquisition

Appendix 8: Audit Trail (If Equipped)