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8. Pipetting Techniques
Two basic pipetting techniques, forward and reverse pipetting, are
associated with Proline
®
Plus pipettes. Forward pipetting is the most
common used pipetting technique. The technique employs the blow-
out function ensuring complete delivery of the liquid. Reverse pipetting
is recommended for highly viscous, biological or foaming liquids, or very
small volumes of liquid. A selected volume plus an excess is aspirated
into the tip. The delivery is done without blow-out, and, thus, the excess
volume remains in the tip. The reverse technique also facilitates the
repeated delivery of the same volume. If reverse pipetting technique is
used the pipette might need recalibration.
8.1. Forward pipetting
1. Fit the tip onto the pipette tip cone.
2. Press the operating button to the first stop.
3. Place the tip just under the surface of the liquid (2-3 mm) and
smoothly release the operating button up to the starting position.
Wait one second. Carefully withdraw the tip from the liquid, touching
against the edge of the container to remove excess from the outside
of the tip.
4. Dispense the liquid by pressing the operating button to the first stop.
After a short delay press the operating button to the second stop.
This action will empty the tip.
8.2. Reverse pipetting
1. Fit the tip onto the pipette tip cone.
2. Press the operating button all the way to the second stop.
3. Place the tip just under the surface of the liquid (2-3 mm) and
smoothly release the operating button up to the starting position.
4. Withdraw the tip from the liquid touching against the edge of the
container to remove excess.
5. Press the operating button smoothly to the first stop to deliver the
desired volume. Hold the operating button at the first stop. The
liquid that remains in the tip should not be included in the delivery.
6. Discard the remaining liquid by pressing the operating button to the
second stop.
8.3. Repetitive reverse technique
1. Follow the reverse technique steps 1 to 5.
2. Continue pipetting by repeating steps 3 to 5 as long as needed.
3. Finally discard the remaining liquid by pressing the operating button
to the second stop.
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