8
9. Recommendations for Good
Pipetting
Make sure that the tip is firmly attached to the tip cone.
Hold the pipette vertically when aspirating the liquid and place the tip
only a few millimeters into the liquid.
Always control the operating button slowly and smoothly.
Pre-rinse the tip before aspirating the liquid by filling and emptying the
tip for three to five times. This is important especially when pipetting
liquids with a viscosity and density greater than water or liquids with
high vapor pressure (e.g. ethanol).
Check that the pipette, tip and liquid are at the same temperature.
When pipetting liquids with temperatures different to the ambient
temperature change the tip after each pipetting. Do not pre-rinse the tip.
To avoid contamination, do not rest the pipette on its side especially
with the tip attached.
Change the tip cone filter regularly (recommendation after 50 - 250
pipetting cycles).
Never strike the tip cone against the tip tray when mounting the tip as
this can damage the pipette.
Avoid rough handling and do not drop the pipette.
Avoid exposing the unit to extreme temperature changes, humidity
and dust (operating temperature from 15°C to 40°C).
10. Maintenance
Proline
®
Plus pipettes have been designed for easy in-house service.
If the pipette is used daily it is recommended to clean|decontaminate
and check the performance of the pipette every three months. Sartorius
also provides complete repair and recalibration service for your pipette
including a service report and performance certificate.
Note: Please make sure that the pipette has been decontaminated
before you send it to us or your local representative. Please advise if any
hazardous material has been used with your pipette.
Note: The use of the tip cone filters may prolong the service interval.
Change the filters regularly.
Note: It is recommended to always use gloves when cleaning the
pipette.
10.1. Daily Cleaning of The Outer Surface of
The Pipette
Your Proline
®
Plus pipette should be checked every day for cleanliness.
To clean and decontaminate the outer surface of your pipette use
ethanol (70%), isopropanol (60%) or a mild detergent and a soft lint-free
cloth.
Gently clean the surface of the pipette with moistened cloth and wipe
dry. Pay special attention to the tip cone. Change the tip cone filter if
needed (Chapter 7.3.)
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