The SINNOWA BS3000S is an optical multi-test analyzer designed for both Biochemistry and Specific Protein test functions. Its optical system incorporates both transmitted light colorimetric detection and scattering light detection, enabling a wide range of multi-test capabilities.
Configuration and Structure:
The BS3000S primarily consists of a Control System (featuring a single-chip microcomputer and touch screen), a Samples and Reagent Incubation system, an Optical and Measuring System, a Peristaltic Pump Suction system, and a Built-in Thermal Printer. Key components visible on the device include a touch screen for interaction, an incubator for samples and reagents, a suction probe for sample aspiration, and a PUSH button for various operations.
Important Technical Specifications:
- Wavelength of Transmitted Light:
- Standard configuration: 340nm, 405nm, 492nm, 510nm, 546nm, 578nm, 620nm.
- Optional: Other wavelengths are available upon request within the range of 340nm to 810nm.
- Wavelength of Scattering Light:
- Standard configuration: 670nm, 840nm.
- Optional: Other wavelengths are available upon request.
- Light Source: 6V, 10W halogen lamp, 2 LEDs.
- Absorbency Range: 0.000 to 3.500 OD.
- Temperature for Reaction Cuvette: 37°C.
- Test Plate Configuration: Supports both a flow cell and reaction cuvettes for Specific protein tests, allowing users to choose as needed.
- Analytical Methods:
- Photometry: End point, fixed time, kinetic, multi-point, serum blank.
- Nephelometry: End point Nephelometry, Kinetic nephelometry.
- Interference Light: ≤2.9A.
- Linearity of Absorbency:
- Absorbance within 0.200–≤0.500: bias should be within ±5%.
- Absorbance within 0.500–≤1.000: bias should be within ±4%.
- Absorbance within 1.000–≤1.800: bias should be within ±2%.
- Stability of Absorbency: Less than 0.002A within 20 minutes at 340nm.
- Repetition of Absorbency: Coefficient variance (CV) ≤1.0%.
- Cross Contamination Rate: When reaction liquid volume is 1ml, the cross contamination rate should be less than 1% by flow cell test; no cross contamination by using separated cuvette inspection.
Installation Requirements:
- Space Requirement: Maintain at least 100mm distance from walls and other objects on all sides (left, right, and back). Ensure sufficient space for distilled water barrels and waste containers.
- Environmental Requirement:
- Working Temperature: 15°C ~ 32°C.
- Working Humidity: 30% ~ 70%.
- Working Atmospheric Pressure: 860hPa ~ 1060hPa.
- Power Supply: 220V~ ±10%, 50Hz±1 Hz.
- Fuse: F2AL250V, 5x20mm.
- Input Power: 150VA.
- A good grounding socket within 1 meter of the equipment is essential.
- The environment should be quiet, clean, and free from dust, noise, and power interference.
- Keep the equipment away from devices like centrifuges, CT scanners, X-ray machines, and sources of radio interference.
- Avoid direct sunlight, ultraviolet rays, and proximity to hot or cool sources, or air conditioning outlets.
- Attention: The power supply socket should be within 1 meter for timely plug removal in case of accidents. Verify that the network voltage matches the equipment voltage and that the power supply is stable (voltage change within 10%).
Usage Features:
- Operation: The device is operated via a touch screen interface. After switching on, the screen displays "BS3000S Multitest Analyzer". Clicking the screen leads to the "Test Selection" interface, offering "Biochemical" and "Specific Protein" options.
- Biochemical Test Functions:
- Main Interface Options: Test, Edit, Result, Wash, Feed, Pump, Gain, Filter, Setup, Reserved.
- Test: Select biochemical items for sample testing; results are displayed and printed automatically.
- Edit: Add, modify, delete, and print biochemical items.
- Result: Print, delete, manage QC, view QC statistics, and print general reports.
- Wash: Shortcut key for cleaning; aspirates 1.5ml per keystroke to clean the flow cell.
- Feed: Used for loading, passing, and cutting printer paper.
- Pump: Calibration for peristaltic pump aspiration volume.
- Gain (AD Auto Zero): Confirms if the instrument is in regular test status.
- Filter: Measures and adjusts each filter's AD value, blank value, and absorbance.
- Setup: Configures cuvette temperature, filter number, cuvette diameter, language, aspiration mode, sleep mode, hospital and instrument model title, time format, date/time, and screen brightness.
- Parameters Settings (Temperature Display): Allows confirmation of temperature accuracy. Calibration temperature can be input by professionals using password "123456".
- Pump Calibration: The default aspiration volume is 3000ul corresponding to 20000 motor steps. If aspiration volume is incorrect, calibration is necessary. Users input the desired volume, aspirate distilled water, and the instrument displays motor steps.
- Instrument Operation Key Process:
- Preheating: Connect to power and turn on the switch; the instrument requires 10-30 minutes of preheating.
- Pipeline Washing: Clean the flow cell before use by inserting the pipette into distilled water and pressing "wash" 5-10 times.
- AD Auto Zero: Select "AD Auto Zero" from the main menu. Insert the aspiration pipe into distilled water, press "PUSH", then "Continue". The instrument will zero, displaying Gain coverage, AD value, and offset value. Alarms will sound if values are out of range. This step is crucial and should be performed at each instrument startup. For flow cell AD auto zero, more distilled water is recommended to prevent bubbles. For cuvette AD auto zero, distilled water should reach more than 10mm from the bottom.
- Specific Proteins Test Function:
- Principle: Based on the light scattering principle. The intensity of scattered light at 90 degrees is directly proportional to the amount and size of immune complexes within certain limits.
- Operation: Choose "Specific protein" from the main menu. Use scattered light for testing. Macro cuvettes or micro cuvettes with adaptors should be used in test pots. Flowing cuvettes are not available for this test. Light-shielding upon cuvettes is required for stable detection signals. The function keys are similar to the "Biochemical" menu.
Maintenance Features:
- Daily Maintenance: Primarily involves washing the flowing cuvette. 10 washes are necessary before testing, and 4-5 washes after each test. If bubbles are present, draw ethanol to soak and flush, then wash with distilled water.
- Weekly Maintenance: Wash the flowing cuvette with detergent. Allow detergent to rest in the cuvette for 5-10 minutes before draining, then flush repeatedly with distilled water. Recommended detergents include 20% sodium hypochlorite solution, 95% absolute ethanol, or dedicated chemistry analyzer detergent.
- Monthly Maintenance: Clean dust and stains from the casing, and correct the sample absorption amount of the peristaltic pump.
- Other Maintenance Items: Use coagulation cuvettes and micro detector cuvettes for "Coagulation" and "Specific Protein" tests. Cuvettes and magnetic rods should be original Sinnowa parts to avoid incorrect results.
- Troubleshooting: The manual provides solutions for common malfunctions.
- Warnings: Always turn off the analyzer, cut off power, and remove the power plug before any repair work. Repairs must be performed by SINNOWA professional trained personnel. Use only the specified power supply and voltage to avoid damage not covered by warranty.
- Caution: Incorrect test results may occur with instrument malfunction. Troubleshoot any detected faults before use. Samples, quality control samples, calibration samples, and waste liquid pose potential biochemistry risks. Operators must comply with laboratory safety regulations (wear PPE like protective clothing, gloves) and local government regulations for waste disposal.
- Malfunction Phenomenon and Maintenance:
- Mistakes with Auto Zero: Possible causes include no distilled water in cuvettes, air bubbles, wrongly connected/leaking/blocked pipette, peristalsis pump hitch, aging/damaged filter, or burned-out instrument bulb.
- Wrong Result or Bad Repetition: Possible causes include air bubbles in cuvettes, improperly installed/leaking peristalsis pump pipe, abnormal aspiration (recalibrate pump), unstable voltage (connect regulated power), hemolytic sample, or invalid reagent.
- Instrument Does Not Work: Cause: Fuse burn-out or poor connection behind the power interface. Maintenance: Replace fuse, check interface.
- Screen Definition Changes: Cause: Local AC voltage difference or LED display voltage variation. Maintenance: Open the instrument, locate the main board cable connected to the display, find the blue 203 potentiometer, and adjust it to change the definition.
- Printer Keeps Walking: Cause: Loose cable connecting printer head to control panel. Maintenance: Open the instrument, remove the printer head, and compress the interface. Attention: Be careful not to drag the white cable and printer head cable too hard.
- Long Heating Time: Cause: Ambient temperature (especially in winter, increase indoor temperature to 10℃~30℃) or insufficient heating voltage. Maintenance: Test incubator heating rod voltage with a digital multimeter; replace if no voltage.
- Slightly Long Testing Time: Cause: Insufficient incubation time for plasma (serum) and reagent, or instrument needs recalibration. Maintenance: Ensure plasma (serum) and reagent incubate for at least 3 minutes (5 minutes in winter).
- Corrections and Replacements to Common Parts:
- Attention: Users must be trained by Sinnowa professional engineers before performing maintenance and replacements independently.
- Replacement of Fuse: Turn off power, pull out power cord, remove power wire from mainframe socket, elicit fuse housing, replace fuse, and plug housing back. Warning: Use only appointed specification fuses.
- Replacement to Light Source: The instrument has a halogen lamp and two LED light sources. Replace if damaged or after 2 years. Steps: Turn off analyzer for 15 minutes, open analyzer, unload cuvettes pallet, unplug lamp power plugin, unscrew and remove lamp, fit new lamp, loosen screws on lamp bracket, turn on power, enter A/D signal detection interface, select wavelength, and adjust lamp position for maximum signal value. For LED replacement (scattering detection abnormal), check or replace the LED light source. Attention: After changing lamp/LED, ensure secure connections and calibrate before normal use. Warnings: Turn off power before replacing lamp. Wait for temperature to decrease as it is very hot. Do not touch the new lamp surface; fingerprints or stains can be removed with rubbing alcohol.
- Replacement to Peristalsis Pump: Open instrument side cover, pull out two pump pipes, take coarse joint from set screws, pull out pump, insert new peristalsis pump, and connect pump pipe as shown in Figure 4. Attention: Be careful when unplugging pump pipe to prevent breaking incoming and outlet points. Inspect peristaltic pump pipe monthly for reliability.
- Replacement to Printing Paper: Open printer cover, remove old paper, load new paper into slot, cut paper port flat, put paper to formfeed, push tight, press FEED, cover printer cover. Attention: Replacement of pump pipe must be provided by SINNOWA; do not use other types.
Transportation and Storage:
- Transportation: Must comply with order contract regulations. Keep away from toxic, harmful, corrosive substances. Prevent severe shocks, rain, and exposure. Overturning is not permitted.
- Storage: Store in an environment temperature of -5°C ~ 50°C, relative humidity no more than 80%, in a well-ventilated indoor area. Do not store with toxic, harmful, or corrosive materials.
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