Thermo Scientific Applied Biosystems QuantStudio 6 Pro - Prepare a Calibration Taqman Array Card

Prepare a calibration TaqMan
™
Array Card
Required materials to prepare calibration TaqMan
™
Array Card
Unless otherwise indicated, all materials are available through thermofisher.com. "MLS" indicates that
the material is available from fisherscientific.com or another major laboratory supplier.
Item Source
Calibration kit
ViiA
™
7 Array Card Spectral Calibration Kit, includes the following
components:
•
Empty TaqMan
™
Array Cards
•
FAM
™
dye
•
VIC
™
dye
•
ROX
™
dye
•
ROI dye mix
•
Background buer
•
FAM
™
/ROX
™
dye
•
VIC
™
/ROX
™
dye
4432314
Equipment and consumables
Centrifuge with custom buckets and card holders, one of the following:
•
Sorvall
™
centrifuge
•
Multifuge
™
centrifuge
•
Megafuge
™
centrifuge
Contact your local sales oce
TaqMan
™
Array Card Sealer Contact your local sales oce
Blank balance TaqMan
™
Array Cards Contact your local sales oce
Pipette, 200 µL with tips MLS
Permanent marker or pen MLS
Powder‑free gloves MLS
Safety glasses MLS
Fill the calibration TaqMan
™
Arr
ay Cards
For detailed instructions to prepare a TaqMan
™
Array Card, see Appendix C, “Detailed procedures for
preparation of a TaqMan
™
Array Card”.
IMPORTANT! W
ear powder-free gloves while creating the calibration array cards.
Chapter 7 Calibrate and verify instrument performance
Perform ROI/uniformity, background, dye, and normalization calibrations for the TaqMan
™
Array Card block
7
98
QuantStudio
™
6 Pro Real-Time PCR System and QuantStudio
™
7 Pro Real-Time PCR System User Guide