Dionex AXP/AXP-MS Manual
2.5.5 Priming the Pump and the Flushing Lines
Connect a syringe to the priming adapter. Run the pump at a flow rate
of 3.00 to 5.00 mL/min. Prime the pump by pulling mobile phase and
any air bubbles through the system and into the syringe (a minimum of
20 mL).
To prime the flush lines for a self-flush head, simply place the inlet
line in the flush solution and connect a syringe to the outlet line and
apply suction until the line is filled with flush solution. Place the outlet
line in the flush solution. Secure both flush lines in the flush solution
container so they stay immersed during pump operation.
2.5.6 Long-Term Pressure Calibration Accuracy
This note applies if your pump is equipped with an electronic pressure
transducer. The transducer has been zeroed and calibrated at the
factory. Over the life of the pump, some drift may occur. For example,
it is typical for the zero to drift <10 psi after about one year of
operation (i.e., with no backpressure on the pump, a reading of 1 to 9
psi may be displayed). A similar drift may also occur at higher
pressures, and is typically less than 1% (e.g., <50 psi at 6000 psi
backpressure).
If pressure calibration and/or drift is a concern, contact Technical
Support for Dionex products. The pump can be shipped back for
recalibration. Alternatively, written calibration and zero-reset
procedures are available. To obtain a copy of these instructions,
contact Technical Support for Dionex products.
2.6 Preparation for Storage or Shipping
2.6.1 Isopropanol Flush
Disconnect the outlet tubing from
the pump. Place the inlet filter in
isopropanol. Use a syringe to draw a minimum of 50 mL through the
pump. Pump a minimum of 5 mL of isopropanol to exit. Leave the
inlet tubing connected to the pump. Place the inlet filter in a small
plastic bag and attach it to the tubing with a rubber band. Plug the
outlet port with the shipping plug, leave a length of outlet tubing on
the pump, or cover the outlet port with plastic film.
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