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Thermo Scientific Varioskan - User Manual

Thermo Scientific Varioskan
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Thermo Scientific
SkanIt
®
Software for
Varioskan
®
version 2.4.3
User Manual
Rev. 3.1

Table of Contents

Question and Answer IconNeed help?

Do you have a question about the Thermo Scientific Varioskan and is the answer not in the manual?

Summary

About the User Manual

Intended users

Describes the intended audience for the manual.

Warning and other markings

Explains symbols and markings used throughout the manual for safety and guidance.

Chapter 1 Introduction

SkanIt Software

Overview of SkanIt Software's purpose and capabilities.

Varioskan system

Description of the Thermo Scientific Varioskan spectral scanning multimode reader.

Chapter 2 Installing SkanIt Software

Before installation

Instructions and notes to consider before proceeding with software installation.

Checking the PC requirements

Lists the minimum hardware and software specifications for installing SkanIt Software.

Installing the software

Step-by-step guide for performing the actual SkanIt Software installation.

Uninstalling SkanIt Software

Instructions on how to remove SkanIt Software from the computer.

Chapter 3 User Interface

Navigating in the software

Overview of the main window layout and navigation within SkanIt Software.

Results viewing window

Explanation of how to view measurement and calculation results.

Chapter 4 Sessions

Launching SkanIt Software

Steps to start and log into the SkanIt Software application.

Creating a new session

Guide to creating a new experiment session from scratch.

Exporting and importing sessions

Methods for transferring sessions and runs between databases.

Chapter 5 Executing a Session

Pathlength correction

Explanation of how pathlength correction is applied to absorbance measurements.

Priming dispensers

Procedure for preparing the dispenser system before use.

Chapter 6 Protocols

Editing a protocol

Guide to defining and modifying measurement protocols.

Area definition

How to define the operational area for protocol steps.

Well loops

Using well loops to group and redefine protocol parameters.

Chapter 7 Step Parameters

Photometric measurement

Details the parameters for measuring absorbance.

Using multipoint in a measurement

Defining measurement points within a well.

Dispense

Instructions for using the instrument's dispensing function.

Chapter 8 Plate Layouts

Editing a plate layout

Steps to modify plate layouts, including adding samples.

Adding samples to the plate layout using Fill Wizard

Using the Fill Wizard to efficiently add sample information to a layout.

Creating new groups for the samples

Organizing samples into groups for analysis.

Chapter 10 Data Processing Parameters

Blank subtraction

How to subtract blank values from measurements.

Quantitative curve fit

Creating standard curves for quantitative analysis.

Kinetic calculations

Processing and analyzing kinetic measurement data.

Chapter 11 Results

Raw measurement data

Viewing the raw data obtained from instrument measurements.

Saving measurement and calculation data

Options for saving or exporting experiment data.

Chapter 12 Instrument and Software Reports

Creating instrument reports

Generating status reports for the instrument.

Chapter 13 Settings

Options

General software settings and preferences.

Instrument

Configuring instrument connection and settings.

SkanIt Control Panel

Managing users, groups, and security settings.

Users

Managing user accounts, including adding and modifying them.

Groups

Managing user groups and assigning rights.

Chapter 14 Menus and Action Panels

Menu commands

Description of all menu items available in SkanIt Software.

Action panels

Explanations of the buttons in the various action panels.

Chapter 16 Troubleshooting Guide

Error messages

Lists common error codes, their explanations, and suggested actions.

Warning messages

Lists common warning messages and recommended actions.

Appendix A Administrators

SkanIt Control Panel

Overview of the SkanIt Control Panel for user management.

Users

Managing user accounts, including adding and modifying them.

Appendix B Database maintenance

Backup

How to create backup files of the SkanIt Software database.

Changing the working database

Selecting which database SkanIt Software uses.

Appendix C Drug Discovery Edition with 21 CFR11 Compliance

Electronic signature

Features related to electronic signatures for FDA compliance.

Audit trail

Tracking user operations and changes within the system.

Summary

About the User Manual

Intended users

Describes the intended audience for the manual.

Warning and other markings

Explains symbols and markings used throughout the manual for safety and guidance.

Chapter 1 Introduction

SkanIt Software

Overview of SkanIt Software's purpose and capabilities.

Varioskan system

Description of the Thermo Scientific Varioskan spectral scanning multimode reader.

Chapter 2 Installing SkanIt Software

Before installation

Instructions and notes to consider before proceeding with software installation.

Checking the PC requirements

Lists the minimum hardware and software specifications for installing SkanIt Software.

Installing the software

Step-by-step guide for performing the actual SkanIt Software installation.

Uninstalling SkanIt Software

Instructions on how to remove SkanIt Software from the computer.

Chapter 3 User Interface

Navigating in the software

Overview of the main window layout and navigation within SkanIt Software.

Results viewing window

Explanation of how to view measurement and calculation results.

Chapter 4 Sessions

Launching SkanIt Software

Steps to start and log into the SkanIt Software application.

Creating a new session

Guide to creating a new experiment session from scratch.

Exporting and importing sessions

Methods for transferring sessions and runs between databases.

Chapter 5 Executing a Session

Pathlength correction

Explanation of how pathlength correction is applied to absorbance measurements.

Priming dispensers

Procedure for preparing the dispenser system before use.

Chapter 6 Protocols

Editing a protocol

Guide to defining and modifying measurement protocols.

Area definition

How to define the operational area for protocol steps.

Well loops

Using well loops to group and redefine protocol parameters.

Chapter 7 Step Parameters

Photometric measurement

Details the parameters for measuring absorbance.

Using multipoint in a measurement

Defining measurement points within a well.

Dispense

Instructions for using the instrument's dispensing function.

Chapter 8 Plate Layouts

Editing a plate layout

Steps to modify plate layouts, including adding samples.

Adding samples to the plate layout using Fill Wizard

Using the Fill Wizard to efficiently add sample information to a layout.

Creating new groups for the samples

Organizing samples into groups for analysis.

Chapter 10 Data Processing Parameters

Blank subtraction

How to subtract blank values from measurements.

Quantitative curve fit

Creating standard curves for quantitative analysis.

Kinetic calculations

Processing and analyzing kinetic measurement data.

Chapter 11 Results

Raw measurement data

Viewing the raw data obtained from instrument measurements.

Saving measurement and calculation data

Options for saving or exporting experiment data.

Chapter 12 Instrument and Software Reports

Creating instrument reports

Generating status reports for the instrument.

Chapter 13 Settings

Options

General software settings and preferences.

Instrument

Configuring instrument connection and settings.

SkanIt Control Panel

Managing users, groups, and security settings.

Users

Managing user accounts, including adding and modifying them.

Groups

Managing user groups and assigning rights.

Chapter 14 Menus and Action Panels

Menu commands

Description of all menu items available in SkanIt Software.

Action panels

Explanations of the buttons in the various action panels.

Chapter 16 Troubleshooting Guide

Error messages

Lists common error codes, their explanations, and suggested actions.

Warning messages

Lists common warning messages and recommended actions.

Appendix A Administrators

SkanIt Control Panel

Overview of the SkanIt Control Panel for user management.

Users

Managing user accounts, including adding and modifying them.

Appendix B Database maintenance

Backup

How to create backup files of the SkanIt Software database.

Changing the working database

Selecting which database SkanIt Software uses.

Appendix C Drug Discovery Edition with 21 CFR11 Compliance

Electronic signature

Features related to electronic signatures for FDA compliance.

Audit trail

Tracking user operations and changes within the system.

Thermo Scientific Varioskan Specifications

General IconGeneral
Wavelength Range200-1000 nm
Light SourceXenon flash lamp
SoftwareSkanIt Software
TypeMicroplate Reader
Detection ModesAbsorbance, Fluorescence, Luminescence, Time-Resolved Fluorescence (TRF)
Plate Formats6- to 384-well plates
ConnectivityUSB
Temperature ControlAmbient to 65°C (optional)
Shakingorbital
DispensingOptional reagent injectors
Monochromator Bandwidth5 nm (standard), 1.25 nm (optional)
Detection SystemPMT (Photomultiplier Tube)
Data OutputASCII, Excel
Power Requirements100-240 V, 50/60 Hz

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