Operator’s Manual
Operating Procedure
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Type of samples
VirClia Lotus automatically performs in vitro diagnostics tests on samples such as blood, serum and other biological
fluids.
NOTE: It might be necessary to validate the pipetting of samples with viscosities anomalously high. Consult the Tech-
nical Service for more information.
Storage
The samples must be stored following the instructions given by the manufacturer of the kit.
Identification of samples
VirClia Lotus is equipped with a barcode reader on the external side of the Sample Rotor. The barcoded samples are
identified through the barcode reader. The instructions include the warning not to exchange samples once scanned.
Minimum volume of sample
The minimum volume of sample is the dead volume plus the sample volume necessary to run the assay. Tubes pre-
programed in the VirClia LOTUS software are as stated below:
75mm x 11mm 100mm x 11mm
75mm x 12mm 100mm x 12mm
75mm x 13mm 100mm x 13mm
75mm x 16mm 100mm x 16mm
NOTE: additional tubes exceeding 16 mm of diameter and/or 100 mm high cannot be programmed.
Dead volume
If the sample volume is less than, or equal to, the minimum volume measured by the software it cannot be aspirated
or detected by the pipetting system. The dead volume is < 200 μL for standard test-tubes. This value can be reduced
by using test-tubes with smaller diameters. In this case, the calibration of test-tubes must be performed by technical
staff authorized and trained by VIRCELL S.L.
WARNING: The use of test-tubes different from the ones for which VirClia Lotus was calibrated may lead to false
negative results due to errors in calculating the samples volumes.
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