VWR M4 - Operation; Photometry

Standard Sample:
10g/L NaI solution or equivalent filter (220nm), 50g/L NaNo
2
solution
or equivalent filter (340 or 360nm)
Measurement:
1. Remove something in the measurement channel, close the sample
chamber cover, press the wavelength value, type in the wavelength of
measurement;
2. Put the Reference in the measurement channel, press the button Zero;
3. Put the Standard Sample in the measurement channel, press the button
Measure, the result is the stray light of this wavelength.
Verifying Noise (0A)
Select Tab Noise (0A) in the Performance verification interface.
Standard Sample:
None
Measurement:
1. Remove something in the measurement channel, close the sample
chamber cover, press the wavelength value, type in the wavelength 500,
press the button Zero;
2. Press the button Measure, the result is the noise of this wavelength.
Verifying Noise (0%T)
Select Tab Noise (0%T) in the Performance verification interface.
Standard Sample:
Block
Measurement:
1. Remove something in the measurement channel, close the sample
chamber cover, press the wavelength value, type in the wavelength 500,
press the button Zero;
2. Put the Block in the measurement channel, press the button Measure,
the result is the dark noise of this wavelength.
Verifying Stability
Select Tab Noise in the Performance verification interface.
Standard Sample:
None
Measurement:
1. Remove something in the measurement channel, close the sample
chamber cover, press the wavelength value, type in the wavelength 500,
press the button Zero;
2. Press the button Measure, the result is the noise of 500nm.
Verifying Bandwidth
Select Tab Dark Bandwidth in the Performance verification interface.
Standard Sample:
Low pressure quartz mercury lamp
Measurement:
1. Open the lamp cover, put the low pressure quartz mercury lamp into the
lamp seat, and turn it on.
2. Remove something in the measurement channel, close the sample
chamber cover, press the wavelength value, type in the wavelength 546.1;
3. Press the button Measure, the result is the bandwidth.
Operation
Important information The cuvettes must be clear and there’s no remains of the
samples on the surface of it. Only Silicon (Quartz) cuvettes are permitted to be used in the range of
UV area.
Photometry
Photometry mode is used to measure the absorbance or transmissivity of the sample.