VWR M4 - Page 45

Three Wavelengths Method： Abs.=A
1
-(WL
1
-WL
2
)*(A
2
-A
3
)/(WL
2
-WL
3
)-A
3
APPENDIX 2
Methods of DNA/Protein Analysis
Method 1： C
DNA
=(A
1
-A
ref
)*f
1
-(A
2
-A
ref
)*f
2
C
Protein
= (A
2
-A
ref
)*f
3
-(A
1
-A
ref
)*f
4
Ratio=(A
1
-A
ref
)/ (A
2
-A
ref
)
A
1
=A
260nm
,A
2
=A
280nm
, A
ref
=A
320nm
(Optional)
f
1
=62.9, f
2
=36.0, f
3
=1552, f
4
=757.3
Method 2： C
DNA
=(A
1
-A
ref
)*f
1
-(A
2
-A
ref
)*f
2
C
Protein
= (A
2
-A
ref
)*f
3
-(A
1
-A
ref
)*f
4
Ratio=(A
1
-A
ref
)/ (A
2
-A
ref
)
A
1
=A
260nm
, A
2
=A
230nm
, A
ref
=A
320nm
(Optional)
f
1
=49.1, f
2
=3.48, f
3
=183, f
4
=75.8